Biotinylated Mouse IL-17A&F Protein (IL7-MM4AFB)
Biotinylated Mouse IL-17A&F Protein (IL7-MM4AFB)
Biotinylated Mouse IL-17A&F Protein (IL7-MM4AFB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse IL-17R alpha, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-17A&F, His Tag with the EC50 of 19.6ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala26-Ala158(IL-17A) & Arg29-Ala161(IL-17F)
Catalog #IL7-MM4AFB
Regular price $525.00
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Product Description
Recombinant Biotinylated Mouse IL-17A&F Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala26-Ala158(IL-17A)&Arg29-Ala161(IL-17F).
Product Background
Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.
Product Category
Recombinant Protein
Target
IL-17A&F
Synonyms
IL-17A;Interleukin-17A;CTLA-8;IL-17;IL-17F;IL24;ML-1;Interleukin-17F
Accession
Species
Mouse
Biotinylated
yes
Amino Acid Range
Ala26-Ala158(IL-17A) & Arg29-Ala161(IL-17F)
Molecular Weight
The protein has a predicted MW of 17.9 kDa (IL-17A) and 14.9 kDa (IL-17F). Due to glycosylation, the protein migrates to 22-25 kDa and 27-30 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Mouse IL-17R alpha, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-17A&F, His Tag with the EC50 of 19.6ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse IL-17R alpha, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-17A&F, His Tag with the EC50 of 19.6ng/ml determined by ELISA.
Biotinylated Mouse IL-17A&F on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Mouse IL-17A&F is greater than 95% as determined by SEC-HPLC.

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