Product Description |
Recombinant Biotinylated Mouse IL-17A&F Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala26-Ala158(IL-17A)&Arg29-Ala161(IL-17F). |
Product Background |
Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines. |
Product Category |
Recombinant Protein |
Target |
IL-17A&F |
Synonyms |
IL-17A;Interleukin-17A;CTLA-8;IL-17;IL-17F;IL24;ML-1;Interleukin-17F |
Accession |
|
Species |
Mouse |
Biotinylated |
yes |
Amino Acid Range |
Ala26-Ala158(IL-17A) & Arg29-Ala161(IL-17F) |
Molecular Weight |
The protein has a predicted MW of 17.9 kDa (IL-17A) and 14.9 kDa (IL-17F). Due to glycosylation, the protein migrates to 22-25 kDa and 27-30 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse IL-17R alpha, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-17A&F, His Tag with the EC50 of 19.6ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Mouse IL-17R alpha, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-17A&F, His Tag with the EC50 of 19.6ng/ml determined by ELISA. |
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Biotinylated Mouse IL-17A&F on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Mouse IL-17A&F is greater than 95% as determined by SEC-HPLC. |
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