• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Rat GPC3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA.
  • Amino Acid Range: Gln25-Met557
Catalog #GPC-RM131
Price
Regular price $000
Regular price Sale price $000
/
Size
Avantor VWR Fisher Scientific

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Rat GPC3/Glypican 3 Protein (GPC-RM131) Summary

Product Description

 Recombinant Rat GPC3/Glypican 3 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln25-Met557.

Product Background

 Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.

Rat GPC3/Glypican 3 Protein (GPC-RM131) Specifications/Details

Protein
 GPC3/Glypican 3
Synonyms
 GPC3; DGSX; Glypican 3; GTR2-2; MXR7; OCI5; OCI-5; SGB; SGBS; SGBS1SDYS; SDYS; SGBS1
Accession
Species
 Rat
Biotinylated
 no
Amino Acid Range
 Gln25-Met557
Molecular Weight
 The protein has a predicted MW of 62.13 kDa. Due to furin-like convertase cleavage site and glycosylation, the protein migrates to 40-45 kDa and 95-200 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Rat GPC3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Rat GPC3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA. Immobilized Rat GPC3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA.
Rat GPC3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Rat GPC3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...