• Express System: E.coli
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Measured in A fluorescence-based N-myristoyltransferase activity assay. The specific activity is >25 pmol/min/µg.
  • Amino Acid Range: Met81-Gln496
Catalog #NMT-RE101
Price
Regular price $39000
Regular price Sale price $39000
/
Size
Avantor VWR Fisher Scientific

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Rat NMT1 Protein (NMT-RE101) Summary

Product Description

 Recombinant Rat NMT1 Protein is expressed from E.coli with His tag at the N-terminus.It contains Met81-Gln496.

Product Background

 N-myristoyltransferase 1 (NMT1) is an indispensable eukaryotic enzyme that catalyses the transfer of myristoyl groups to the amino acid terminal residues of numerous proteins. This catalytic process is required for the growth and development of many eukaryotes and viruses. Elevated expression and activity of NMT1 is observed to varying degrees in a variety of tumour types (e.g. colon, lung and breast tumours). Furthermore, an elevated level of NMT1 in tumours is associated with poor survival. Therefore, a relationship exists between NMT1 and tumours.

Rat NMT1 Protein (NMT-RE101) Specifications/Details

Protein
 NMT1
Accession
Species
 Rat
Biotinylated
 no
Amino Acid Range
 Met81-Gln496
Molecular Weight
 The protein has a predicted MW of 49.16 kDa same as Bis-Tris PAGE result.
Activity
 Measured in A fluorescence-based N-myristoyltransferase activity assay. The specific activity is >25 pmol/min/µg.
Product Tag
 N-His
Fluorophore
 Unconjugated
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Rat NMT1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Rat NMT1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rat NMT1 is greater than 95% as determined by SEC-HPLC. The purity of Rat NMT1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...