TnpB Nuclease (CAS-EE151) Summary
Product Description |
Derived from environmental metagenomes, TnpB is one of the smallest known RNA-guided nucleases and serves as a compact member of the TnpB protein family. This nuclease forms a ribonucleoprotein complex (RNP) with its guide RNA (reRNA) to recognize specific DNA motifs (e.g., TTTAA) on the target DNA. Precise sequence targeting is achieved through complementary base pairing between the target DNA and the 3'-terminal sequence (typically 20 nt) of the guide RNA. This interaction induces a site-specific double-strand break (DSB), and gene editing is realized as the cell's endogenous repair mechanisms resolve these breaks. |
Product Category |
Gene Enzyme |
TnpB Nuclease (CAS-EE151) Specifications/Details
Applications |
This system facilitates precise gene knockout and knock-in for diverse fields, including cell and gene therapy, agricultural biotechnology and beyond. |
Concentration |
10.0 mg/ml |
Source |
E.coli |
Molecular Weight |
The enzyme has a predicted MW of 91.51 kDa. |
Expression System |
E.coli |
Purity |
Bis-Tris PAGE purity≥90.0% SEC-HPLC purity≥90.0% |
Endotoxin |
≤ 10.0 EU/mg |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Transport on dry ice. |
Stability And Storage |
Store at -80 ±10°C. Avoid repeated freezing and thawing. |
Validation Images
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In vitro cleavage assays verify that TnpB nuclease effectively cleaves DNA substrates. |
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TnpB Nuclease efficiently knocks out KLKB1, TTR, and TET2 genes in HEK293T cells, as well as the ANGPTL3 gene in Hepa1-6 cells. |
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Analysis of TnpB Nuclease on Bis-Tris PAGE under reducing (R) and non-reducing (N) conditions. Purity is greater than 90.0%. |
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The purity of TnpB Nuclease is confirmed to be greater than 90.0% by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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