• Applications: This system facilitates precise gene knockout and knock-in for diverse fields, including cell and gene therapy, agricultural biotechnology and beyond.
  • Express System: E.coli
  • Purity: Bis-Tris PAGE purity≥90.0% SEC-HPLC purity≥90.0%
Catalog #CAS-EE151
Price
Regular price $2,79400
Regular price Sale price $2,79400
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TnpB Nuclease (CAS-EE151) Summary

Product Description

 Derived from environmental metagenomes, TnpB is one of the smallest known RNA-guided nucleases and serves as a compact member of the TnpB protein family. This nuclease forms a ribonucleoprotein complex (RNP) with its guide RNA (reRNA) to recognize specific DNA motifs (e.g., TTTAA) on the target DNA, enabling the sequence-specific activity of TnpB nuclease. Precise sequence targeting is achieved through complementary base pairing between the target DNA and the 3'-terminal sequence (typically 20 nt) of the guide RNA. This interaction induces a site-specific double-strand break (DSB), and gene editing is realized as the cell's endogenous repair mechanisms resolve these breaks.

TnpB Nuclease (CAS-EE151) Specifications/Details

Applications
 This system facilitates precise gene knockout and knock-in for diverse fields, including cell and gene therapy, agricultural biotechnology and beyond.
Concentration
 10.0 mg/ml
Source
 E.coli
Molecular Weight
 The enzyme has a predicted MW of 91.51 kDa.
Expression System
 E.coli
Purity
 Bis-Tris PAGE purity≥90.0% SEC-HPLC purity≥90.0%
Endotoxin
 ≤ 10.0 EU/mg

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Transport on dry ice.
Stability And Storage
 Store at -80 ±10°C. Avoid repeated freezing and thawing.

Validation Images

In vitro cleavage assays verify that TnpB nuclease effectively cleaves DNA substrates. In vitro cleavage assays verify that TnpB nuclease effectively cleaves DNA substrates.
TnpB Nuclease efficiently knocks out KLKB1, TTR, and TET2 genes in HEK293T cells, as well as the ANGPTL3 gene in Hepa1-6 cells. TnpB Nuclease efficiently knocks out KLKB1, TTR, and TET2 genes in HEK293T cells, as well as the ANGPTL3 gene in Hepa1-6 cells.
Analysis of TnpB Nuclease on Bis-Tris PAGE under reducing (R) and non-reducing (N) conditions. Purity is greater than 90.0%. Analysis of TnpB Nuclease on Bis-Tris PAGE under reducing (R) and non-reducing (N) conditions. Purity is greater than 90.0%.
The purity of TnpB Nuclease is confirmed to be greater than 90.0% by SEC-HPLC. The purity of TnpB Nuclease is confirmed to be greater than 90.0% by SEC-HPLC.
Other Available Proteins:
Finding available proteins...