MaxNuclease, GMP grade (GMP-NUC-SE101)

  • Description: Degrades both DNA and RNA such as double-stranded, single-stranded, linear, circular or supercoiled. No base preference is observed.
  • Applications: Removing DNA/RNA from other biologicals. Reducing viscosity caused by nucleic acids, Purification of viral vaccines, viral vectors for vaccine. Preparing samples in western blot analysis, two-dimensional gel electrophoresis, ELISA and chromatography. Preventing cell clumping.
  • Quality: Manufactured according to GMP guidelines. Accepted by the FDA's Drug Master Files (DMF) database, DMF#036799.

Catalog: GMP-NUC-SE101

$500.00
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Product Description DMF filed- this product has been submitted to FDA's Drug Master Files (DMFs) and is granted the DMF #036799. MaxNuclease is a non-specific nucleic acid endonuclease derived from Serratia Marcescens that degrades both DNA and RNA such as double-stranded, single-stranded, linear, circular or supercoiled. No base preference is observed. MaxNuclease hydrolyzes internal phosphodiester bonds between the nucleotides and completely digests nucleic acids into two to five bases in length.
Application Removing DNA/RNA from other biologicals. Reducing viscosity caused by nucleic acids, Purification of viral vaccines, viral vectors for vaccine. Preparing samples in western blot analysis, two-dimensional gel electrophoresis, ELISA and chromatography. Preventing cell clumping.
Concentration ≥ 250U/µL
Unit Definition One unit is defined as the amount of enzyme required to produce a change in absorbance at 260nm of 1.0 in the time of 30 minutes, under optimum conditions with excess substrate.
Source Expressed in E.coli that carries the nuclease gene from Serratia Marcescens.
Molecular Weight 27.8kDa
Quality Standards Activity (Dissolve herring sperm DNA): ≥ 250 U/µL
Purity (Bis-Tris): ≥ 95%
Purity (SEC-HPLC): ≥ 99%
Residual Protease: Negative
Endotoxin: ≤ 0.01 EU/kU
Residual Host Protein: ≤ 10 ppm
Sterility: Negative
Residual Heavy Metal: ≤ 10 ppm
Mycoplasma: Negative
Shipping Shipped with blue ice
Stability And Storage -20±5°C for 24 months. Avoid repeated freeze-thaw cycles.
Figure 1. The purity of MaxNuclease via Bis-Tris Page is ≥ 95%.
Figure 2. The purity of MaxNuclease via HPLC is ≥ 99%.
Figure 3. MaxNuclease shows comparable degradation activity of nucleic acids to leading competitors.

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