SDS-PAGE image showing purified protein band with molecular weight marker for validation of sample quality and expression level.
  • Description: Degrades both DNA and RNA such as double-stranded, single-stranded, linear, circular or supercoiled. No base preference is observed.
  • Applications: Removing DNA/RNA from other biologicals. Reducing viscosity caused by nucleic acids, Purification of viral vaccines, viral vectors for vaccine. Preparing samples in western blot analysis, two-dimensional gel electrophoresis, ELISA and chromatography. Preventing cell clumping.
  • Quality: FDA Drug Master Files #036799
    Manufactured according to GMP guidelines.
Catalog #GMP-NUC-SE101
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MaxNuclease, GMP-Grade (GMP-NUC-SE101), DMF #036799 Summary

Product Description

 MaxNuclease is a non-specific nucleic acid endonuclease derived from Serratia Marcescens that degrades both DNA and RNA such as double-stranded, single-stranded, linear, circular or supercoiled. No base preference is observed. MaxNuclease hydrolyzes internal phosphodiester bonds between the nucleotides and completely digests nucleic acids into two to five bases in length.

This product has been filed with the FDA Drug Master Files (DMF) and is assigned DMF #036799.

MaxNuclease, GMP-Grade (GMP-NUC-SE101), DMF #036799 Specifications/Details

Applications

 Removing DNA/RNA from other biologicals. Reducing viscosity caused by nucleic acids, Purification of viral vaccines, viral vectors for vaccine. Preparing samples in western blot analysis, two-dimensional gel electrophoresis, ELISA and chromatography. Preventing cell clumping.

Concentration

 ≥ 250U/µL

Unit Definition

 One unit is defined as the amount of enzyme required to produce a change in absorbance at 260nm of 1.0 in the time of 30 minutes, under optimum conditions with excess substrate.

Source

 Expressed in E.coli that carries the nuclease gene from Serratia Marcescens.
Biotinylated
 no
Molecular Weight
 27.8kDa

Quality Standards

 Activity (Dissolve herring sperm DNA): ≥ 250 U/µL
Purity (Bis-Tris): ≥ 95%
Purity (SEC-HPLC): ≥ 99%
Residual Protease: Negative
Endotoxin: ≤ 0.01 EU/kU
Residual Host Protein: ≤ 10 ppm
Sterility: Negative
Residual Heavy Metal: ≤ 10 ppm
Mycoplasma: Negative

Shipping, Reconstitution, & Storage

Shipping
 Shipped with blue ice
Stability And Storage
 -20±5°C for 36 months. Avoid repeated freeze-thaw cycles.

Validation Images

Figure 1. The purity of MaxNuclease via Bis-Tris Page is ≥ 95%. Figure 1. The purity of MaxNuclease via Bis-Tris Page is ≥ 95%.
Figure 2. The purity of MaxNuclease via HPLC is ≥ 99%. Figure 2. The purity of MaxNuclease via HPLC is ≥ 99%.
Figure 3. MaxNuclease shows comparable degradation activity of nucleic acids to leading competitors. Figure 3. MaxNuclease shows comparable degradation activity of nucleic acids to leading competitors.
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