MaxNuclease, GMP grade (GMP-NUC-SE101)
Catalog: GMP-NUC-SE101
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| Product Description | DMF filed- this product has been submitted to FDA's Drug Master Files (DMFs) and is granted the DMF #036799. MaxNuclease is a non-specific nucleic acid endonuclease derived from Serratia Marcescens that degrades both DNA and RNA such as double-stranded, single-stranded, linear, circular or supercoiled. No base preference is observed. MaxNuclease hydrolyzes internal phosphodiester bonds between the nucleotides and completely digests nucleic acids into two to five bases in length. |
| Application | Removing DNA/RNA from other biologicals. Reducing viscosity caused by nucleic acids, Purification of viral vaccines, viral vectors for vaccine. Preparing samples in western blot analysis, two-dimensional gel electrophoresis, ELISA and chromatography. Preventing cell clumping. |
| Concentration | ≥ 250U/µL |
| Unit Definition | One unit is defined as the amount of enzyme required to produce a change in absorbance at 260nm of 1.0 in the time of 30 minutes, under optimum conditions with excess substrate. |
| Source | Expressed in E.coli that carries the nuclease gene from Serratia Marcescens. |
| Molecular Weight | 27.8kDa |
| Quality Standards | Activity (Dissolve herring sperm DNA): ≥ 250 U/µL Purity (Bis-Tris): ≥ 95% Purity (SEC-HPLC): ≥ 99% Residual Protease: Negative Endotoxin: ≤ 0.01 EU/kU Residual Host Protein: ≤ 10 ppm Sterility: Negative Residual Heavy Metal: ≤ 10 ppm Mycoplasma: Negative |
| Shipping | Shipped with blue ice |
| Stability And Storage | -20±5°C for 12 months. Avoid repeated freeze-thaw cycles. |
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