MaxNuclease, GMP-Grade (GMP-NUC-SE101), DMF #036799
Catalog: GMP-NUC-SE101
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| Product Description | MaxNuclease is a non-specific nucleic acid endonuclease derived from Serratia Marcescens that degrades both DNA and RNA such as double-stranded, single-stranded, linear, circular or supercoiled. No base preference is observed. MaxNuclease hydrolyzes internal phosphodiester bonds between the nucleotides and completely digests nucleic acids into two to five bases in length. This product has been filed with the FDA Drug Master Files (DMF) and is assigned DMF #036799. |
| Application | Removing DNA/RNA from other biologicals. Reducing viscosity caused by nucleic acids, Purification of viral vaccines, viral vectors for vaccine. Preparing samples in western blot analysis, two-dimensional gel electrophoresis, ELISA and chromatography. Preventing cell clumping. |
| Concentration | ≥ 250U/µL |
| Unit Definition | One unit is defined as the amount of enzyme required to produce a change in absorbance at 260nm of 1.0 in the time of 30 minutes, under optimum conditions with excess substrate. |
| Source | Expressed in E.coli that carries the nuclease gene from Serratia Marcescens. |
| Molecular Weight | 27.8kDa |
| Quality Standards | Activity (Dissolve herring sperm DNA): ≥ 250 U/µL Purity (Bis-Tris): ≥ 95% Purity (SEC-HPLC): ≥ 99% Residual Protease: Negative Endotoxin: ≤ 0.01 EU/kU Residual Host Protein: ≤ 10 ppm Sterility: Negative Residual Heavy Metal: ≤ 10 ppm Mycoplasma: Negative |
| Shipping | Shipped with blue ice |
| Stability And Storage | -20±5°C for 24 months. Avoid repeated freeze-thaw cycles. |
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Figure 1. The purity of MaxNuclease via Bis-Tris Page is ≥ 95%. |
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Figure 2. The purity of MaxNuclease via HPLC is ≥ 99%. |
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Figure 3. MaxNuclease shows comparable degradation activity of nucleic acids to leading competitors. |
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