Biolayer Interferometry (BLI) for Nanodisc-Displayed Membrane Proteins | Protocol

Download this protocol for instructions on measuring binding kinetics between antibodies and nanodisc-displayed membrane proteins using Biolayer Interferometry (BLI). This protocol is optimized for use with Protein A probes and nanodiscs containing targets such as CCR8 and A2AR, and includes sample data with affinity constants (KD) in the low nanomolar range.