Product Description |
MDA5 dsRNA Quick-Quantification Kit is used to detect the presence of undesired dsRNA molecules in IVT reactions or dsRNA in viral/non-viral nucleic acid extracts. MDA5 is an innate immune receptor for double-stranded RNA (dsRNA) with dsRNA-dependent ATPase activity. Single-stranded RNA (ssRNA) doesn’t stimulate MDA5 ATPase activity, which enables high specificity for dsRNA detection. Upon dsRNA binding, MDA5 ATPase is activated, converting ATP into ADP+Pi. Then, inorganic phosphate (Pi) reacts with malachite green reagent and forms colored compounds. Colorimetric absorption at 620nm is proportional to dsRNA concentration. |
Form |
Multi |
Shipping |
Kits are shipped with blue ice. |
Stability And Storage |
Store the kit at 2-8°C. The kit remains active for up to 1 year. Opened dsRNA standard may be stored for up to 2 weeks at 2-8°C. It should be stored at -20°C for long-term usage. Avoid repeated freeze-thaw cycles. |
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Example standard curve |
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Example of standard dilution |
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