MDA5 dsRNA Quick-Quantification Kit (MD-DS00B )

  • Description: Detect the presence of undesired dsRNA molecules in IVT reactions or dsRNA in viral/non-viral nucleic acid extracts
  • Applications: Detect the presence of undesired dsRNA molecules in IVT reactions or dsRNA in viral/non-viral nucleic acid extracts.
  • Quality:
    Detection Range: 0.078ng/µL~5ng/µL
    Sensitivity: 0.078ng/µL

Catalog: MD-DS00B

$615.00
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Product Description MDA5 dsRNA Quick-Quantification Kit is used to detect the presence of undesired dsRNA molecules in IVT reactions or dsRNA in viral/non-viral nucleic acid extracts. MDA5 is an innate immune receptor for double-stranded RNA (dsRNA) with dsRNA-dependent ATPase activity. Single-stranded RNA (ssRNA) doesn’t stimulate MDA5 ATPase activity, which enables high specificity for dsRNA detection. Upon dsRNA binding, MDA5 ATPase is activated, converting ATP into ADP+Pi. Then, inorganic phosphate (Pi) reacts with malachite green reagent and forms colored compounds. Colorimetric absorption at 620nm is proportional to dsRNA concentration.
Form Multi
Shipping Kits are shipped with blue ice.
Stability And Storage Store the kit at 2-8°C. The kit remains active for up to 1 year. Opened dsRNA standard may be stored for up to 2 weeks at 2-8°C. It should be stored at -20°C for long-term usage. Avoid repeated freeze-thaw cycles.
Example standard curve
Example of standard dilution

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