Mouse Immunization and Hybridoma Screening Using VLP- or Nanodisc-Displayed Membrane Proteins | Protocol

Download this protocol for conducting mouse immunization and hybridoma generation using full-length membrane proteins displayed on virus-like particles (VLPs) or copolymer nanodiscs. Designed for antibody discovery campaigns targeting difficult-to-express transmembrane proteins, this protocol outlines step-by-step procedures for antigen preparation, immunization, ELISA titer screening, hybridoma cell fusion, and monoclonal selection.