Bulk Quote Inquiry

ROR-HM401-100μg / Inquiry

ROR-HM401-500μg / Inquiry

ROR-HM401-1mg / Inquiry

Human/Cynomolgus/Rhesus macaque ROR1 Protein

Product Info
Source

Recombinant Human/Cynomolgus/Rhesus macaque ROR1 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gln30-Glu403.[Accession | Q01973-1]

Molecular Weight

The protein has a predicted MW of 44.9 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human/Cynomolgus/Rhesus macaque ROR1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human/Cynomolgus/Rhesus macaque ROR1 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human/Cynomolgus/Rhesus macaque ROR1 at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-ROR1 Antibody, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA.

Batch to Batch Consistency

Immobilized Human/Cynomolgus/Rhesus macaque ROR1 at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-ROR1 Antibody, hFc Tag with the EC50 of 7.7 / 7.3 / 7.2 ng/ml determined by ELISA.

Background

ROR1 (Receptor tyrosine kinase-like orphan receptor 1), also known as neurotrophic tyrosine kinase receptor-related 1 (NTRKR1),  is a member of  the ROR family within receptor tyrosine kinases (RTK) superfamily. Two ROR family members (ROR1 and ROR2) have been identified and are characterized by the intracellular tyrosine kinase domains, highly related to those of the Trk-family receptor tyrosine kinases, and by the extracellular Frizzled-like cysteine-rich domains and kringle domains, which are common to receptors of the Wnt family members.

Synonyms

Inactive tyrosine-protein kinase transmembrane receptor ROR1;Neurotrophic tyrosine kinase, receptor-related 1;ROR1;NTRKR1

References

(1)Zhang S , Cui B , Lai H , et al. Ovarian cancer stem cells express ROR1, which can be targeted for anti–cancer-stem-cell therapy[J]. Proceedings of the National Academy of Sciences, 2014, 111(48):17266-71.

Kactus Biosystems Co.Ltd
Online Message