Cas9 Nuclease (GMP-CAS-EE110), DMF #036578

  • Description: Recombinant CRISPR Cas9 protein for T-Cells, Stem Cells, etc.

    Derived from E. Coli with CRISPR Cas9 gene of S. pyogenes
  • Applications: Genome editing with CRISPR
  • Quality: FDA Drug Master Files (DMF) #036578, GMP-Grade, Suitable for Manufacturing, Antibiotic-free and animal-free production
  • Related Products:
    Cas9 ELISA Kit (CAS-MM00B)
    AsCas12a (CAS-EE121)

Catalog: GMP-CAS-EE110


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Product Description Recombinant Streptococcus pyogenes Cas9 nuclease, purified from E.coli, is a powerful tool for genome editing. The nuclease can also be applied to the field of molecular diagnosis. This product has undergone codon optimization, nuclear localization signal (NLS) design, and optimization of E. coli expression and purification.

This product has been filed with the FDA Drug Master Files (DMF) and is assigned DMF #036578.
Synonyms CRISPR-associated endonuclease Cas9/Csn1, cas9, SpCas9, SpyCas9
Application Genome editing with CRISPR
Concentration 10 mg/mL
Source E. coli with CRISPR Cas9 gene of S. pyogenes
Quality Standards Concentration: 9.5-12.5mg/mL
Purity (Bis-Tris): ≥95%
Purity (RP-HPLC): ≥95%
Purity (SEC-HPLC): ≥95%
Activity (In Vitro Cleavage Activity): > 85%
Endotoxin: ≤10EU/mg
Residual DNase: ≤LOD
Residual RNase: ≤LOD
Residual Host Protein: ≤100mg/mL
Residual Host Cell DNA: ≤200ng/mL
Sterility: Negative
Mycoplasma: Negative
Form Liquid
Shipping Shipped with dry ice
Stability And Storage -20±5°C for 24 months. Avoid repeated freeze-thaw cycles.
Figure 1. Cas9 cuts substrate DNA standard via in vitro cutting experiment. Results show the cleavage activity of KACTUS Cas9 is equivalent to that of leading competitors.
Figure 2. Cas9 is electrotransfected into 293T cell line in the form of RNP to knock out the target gene. Results show KACTUS Cas9 has the same knockout efficiency as leading competitors.
Figure 3. The purity of KACTUS Cas9 detected by SEC-HPLC is greater than 95%.
Figure 4. The purity of KACTUS Cas9 detected by Tris-Bis-PAGE is greater than 95%.

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