Product Description |
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and Cas9. |
Synonyms |
CRISPR-associated endonuclease Cas9/Csn1, cas9, SpCas9, SpyCas9 |
Applications |
Genome editing with CRISPR |
Concentration |
10 mg/mL |
Source |
E. coli with CRISPR Cas9 gene of S. pyogenes |
Biotinylated |
no |
Quality Standards |
Concentration: 9.5-11.5 mg/mL Activity: ≥ 85.0% Purity(RP-HPLC): ≥ 95.0% Purity(SEC-HPLC):Monomer ≥ 95.0%, Aggregates ≤ 5.0% Purity(NR-CE):≥ 85.0% Purity(R-CE):≥ 90.0% Residual DNase: Negative Residual RNase: Negative Residual Host Cell Protein: ≤ 100.0 ng/mL Residual Host Cell DNA: ≤ 3.0 ng/mL Endotoxin: ≤ 10.0 EU/mg Sterility: No growth Residual Nickel Salt: ≤ 10.0 ppm pH: 7.4±0.5 |
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Stability And Storage |
-20±5°C for 18 months. Avoid repeated freeze-thaw cycles. |
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Figure 1. Cas9 cuts substrate DNA standard via in vitro cutting experiment. Results show the cleavage activity of KACTUS Cas9 is equivalent to that of leading competitors. |
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Figure 2. Cas9 is electrotransfected into 293T cell line in the form of RNP to knock out the target gene. Results show KACTUS Cas9 has the same knockout efficiency as leading competitors. |
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Figure 3. The purity of KACTUS Cas9 detected by SEC-HPLC is greater than 95%. |
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Figure 4. The purity of KACTUS Cas9 detected by Tris-Bis-PAGE is greater than 95%. |
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