Cas9 Nuclease (GMP-CAS-EE110), DMF #036578
|Product Description||Recombinant Streptococcus pyogenes Cas9 nuclease, purified from E.coli, is a powerful tool for genome editing. The nuclease can also be applied to the field of molecular diagnosis. This product has undergone codon optimization, nuclear localization signal (NLS) design, and optimization of E. coli expression and purification.
This product has been filed with the FDA Drug Master Files (DMF) and is assigned DMF #036578.
|Synonyms||CRISPR-associated endonuclease Cas9/Csn1, cas9, SpCas9, SpyCas9|
|Application||Genome editing with CRISPR|
|Source||E. coli with CRISPR Cas9 gene of S. pyogenes|
|Quality Standards||Concentration: 9.5-12.5mg/mL Purity (Bis-Tris): ≥95% Purity (RP-HPLC): ≥95% Purity (SEC-HPLC): ≥95% Activity (In Vitro Cleavage Activity): > 85% Endotoxin: ≤10EU/mg Residual DNase: ≤LOD Residual RNase: ≤LOD Residual Host Protein: ≤100mg/mL Residual Host Cell DNA: ≤200ng/mL Sterility: Negative Mycoplasma: Negative|
|Shipping||Shipped with dry ice|
|Stability And Storage||-20±5°C for 24 months. Avoid repeated freeze-thaw cycles.|