Cas9 Nuclease (GMP-CAS-EE110), DMF #036578
Catalog: GMP-CAS-EE110
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| Product Description | Recombinant Streptococcus pyogenes Cas9 nuclease, purified from E.coli, is a powerful tool for genome editing. The nuclease can also be applied to the field of molecular diagnosis. This product has undergone codon optimization, nuclear localization signal (NLS) design, and optimization of E. coli expression and purification. This product has been filed with the FDA Drug Master Files (DMF) and is assigned DMF #036578. |
| Synonyms | CRISPR-associated endonuclease Cas9/Csn1, cas9, SpCas9, SpyCas9 |
| Application | Genome editing with CRISPR |
| Concentration | 10 mg/mL |
| Source | E. coli with CRISPR Cas9 gene of S. pyogenes |
| Quality Standards | Concentration: 9.5-12.5mg/mL Purity (Bis-Tris): ≥95% Purity (RP-HPLC): ≥95% Purity (SEC-HPLC): ≥95% Activity (In Vitro Cleavage Activity): > 85% Endotoxin: ≤10EU/mg Residual DNase: ≤LOD Residual RNase: ≤LOD Residual Host Protein: ≤100mg/mL Residual Host Cell DNA: ≤200ng/mL Sterility: Negative Mycoplasma: Negative |
| Form | Liquid |
| Shipping | Shipped with dry ice |
| Stability And Storage | -20±5°C for 24 months. Avoid repeated freeze-thaw cycles. |
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Figure 1. Cas9 cuts substrate DNA standard via in vitro cutting experiment. Results show the cleavage activity of KACTUS Cas9 is equivalent to that of leading competitors. |
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Figure 2. Cas9 is electrotransfected into 293T cell line in the form of RNP to knock out the target gene. Results show KACTUS Cas9 has the same knockout efficiency as leading competitors. |
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Figure 3. The purity of KACTUS Cas9 detected by SEC-HPLC is greater than 95%. |
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Figure 4. The purity of KACTUS Cas9 detected by Tris-Bis-PAGE is greater than 95%. |
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