CRISPR Cas9 Protein, GMP grade (GMP-CAS-EE109)
  • Description: Recombinant CRISPR Cas9 protein ideal for stem cells (i.e. thalassemias), T-cells, etc.
  • Applications: Genome editing with CRISPR
  • Quality: GMP-Grade, Suitable for Manufacturing, Antibiotic-free and animal-free production
Catalog #GMP-CAS-EE109
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Product Description

 CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and Cas9.

Synonyms

 CRISPR-associated endonuclease Cas9/Csn1, cas9, SpCas9, SpyCas9

Applications

 Genome editing with CRISPR

Concentration

 10 mg/mL

Source

 E. coli with CRISPR Cas9 gene of S. pyogenes

Biotinylated

 no

Quality Standards

 Concentration: 9.5-11.5 mg/mL
Activity: ≥ 85.0%
Purity(RP-HPLC): ≥ 95.0%
Purity(SEC-HPLC):Monomer ≥ 95.0%, Aggregates ≤ 5.0%
Purity(NR-CE):≥ 85.0%
Purity(R-CE):≥ 90.0%
Residual DNase: Negative
Residual RNase: Negative
Residual Host Cell Protein: ≤ 100.0 ng/mL
Residual Host Cell DNA: ≤ 3.0 ng/mL
Endotoxin: ≤ 10.0 EU/mg
Sterility: No growth
Residual Nickel Salt: ≤ 10.0 ppm
pH: 7.4±0.5
Form
 Liquid
Shipping
 Shipped with dry ice.
Stability And Storage
 -20±5°C for 18 months. Avoid repeated freeze-thaw cycles.
Figure 1. Cas9 cuts substrate DNA standard via <em>in vitro</em> cutting experiment. Results show the cleavage activity of KACTUS Cas9 is equivalent to that of leading competitors. Figure 1. Cas9 cuts substrate DNA standard via in vitro cutting experiment. Results show the cleavage activity of KACTUS Cas9 is equivalent to that of leading competitors.
Figure 2. Cas9 is electrotransfected into 293T cell line in the form of RNP to knock out the target gene. Results show KACTUS Cas9 has the same knockout efficiency as leading competitors. Figure 2. Cas9 is electrotransfected into 293T cell line in the form of RNP to knock out the target gene. Results show KACTUS Cas9 has the same knockout efficiency as leading competitors.
Figure 3. The purity of KACTUS Cas9 detected by SEC-HPLC is greater than 95%. Figure 3. The purity of KACTUS Cas9 detected by SEC-HPLC is greater than 95%.
Figure 4. The purity of KACTUS Cas9 detected by Tris-Bis-PAGE is greater than 95%. Figure 4. The purity of KACTUS Cas9 detected by Tris-Bis-PAGE is greater than 95%.
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