Name: Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer Protein (MHC-HM407B)
Brand: KactusBio
SKU: MHC-HM407B-100UG
Price: 310.0 USD
Availability: InStock

Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer Protein (MHC-HM407B)

Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer, His Tag captured on CM5 Chip via Anti-His Antibody can bind HLA-A*02:01&B2M&AFP TCR with an affinity constant of 0.14 uM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-A*02:01&B2M&AFP (FMNKFIYEI) Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA (QC Test).

Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-A*02:01&B2M&AFP (FMNKFIYEI) Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.14 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and FMNKFIYEI peptide

Catalog #MHC-HM407B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human HLA-A02:01&B2M&AFP (FMNKFIYEI) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A02:01), Ile21-Met119(B2M) and FMNKFIYEI peptide.
Product Background	Alpha-fetoprotein (AFP), a specific liver cancer marker, T cells expressing AFP-CAR selectively degranulated, released cytokines, and lysed liver cancer cells that were HLA-A*02:01 /AFP while sparing cells from multiple tissue types that were negative for either expressed proteins.CAR T-cell immunotherapy targeting intracellular/secreted solid tumor antigens can elicit a potent antitumor response.
Product Category	Recombinant Protein

Protein	HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer
Synonyms	Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-feto; AFP; HPAFP; AFPD; FETA; MHC
MHC Form	Monomer
Allele	HLA-A*02:01
Accession	A0A140T913(HLA-A*02:01)&P61769(B2M)&FMNKFIYEI
Species	Human
Biotinylated	yes
Amino Acid Range	Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and FMNKFIYEI peptide
Molecular Weight	The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human HLA-A02:01&B2M&AFP (FMNKFIYEI) Monomer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-A02:01&B2M&AFP (FMNKFIYEI) Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.14 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Biotinylated Human HLA-A02:01&B2M&AFP (FMNKFIYEI) Monomer, His Tag captured on CM5 Chip via Anti-His Antibody can bind HLA-A02:01&B2M&AFP TCR with an affinity constant of 0.14 uM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human HLA-A02:01&B2M&AFP (FMNKFIYEI) Monomer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-A02:01&B2M&AFP (FMNKFIYEI) Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA (QC Test).
	Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human HLA-A*02:01&B2M&AFP (FMNKFIYEI) Monomer is greater than 95% as determined by SEC-HPLC.