Regular price
$310.00
Order now, ships by:
Product Description |
Recombinant Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01) and Ile21-Met119(B2M). |
Product Background |
HLA-A*02:01&B2M&Peptide ready Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*02:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner. |
Product Category |
Recombinant Protein |
Protein |
Peptide Ready HLA-A*02:01&B2M Monomer |
Synonyms |
MHC; HLA-A*02:01; Peptide Ready |
MHC Form |
Monomer |
Allele |
HLA-A*02:01 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gly25-Thr305(HLA-A*02:01) & Ile21-Met119(B2M) |
Molecular Weight |
The protein has a predicted MW of 48.10 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA. |
|
|
Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer is greater than 95% as determined by SEC-HPLC. |
Additional Information
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
More from MHC Complexes
Recently viewed
