Human TNFSF15 Trimer Protein (FSF-HM415)

  • Express System: HEK293
  • Product Tag: N-His-Flag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human TNFSF15 Trimer, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 10.2ng/ml determined by ELISA. The affinity constant of 3.06 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Asp91-Leu251

Catalog: FSF-HM415

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Product Description Recombinant Human TNFSF15 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Asp91-Leu251.

Product Background

TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.

Product Category

Recombinant Protein

Target

TNFSF15 trimer
Synonyms TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A

Gene/Protein ID

O95150-1

Species

Human

Exact Sequence

Asp91-Leu251
Molecular Weight The protein has a predicted MW of 58.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human TNFSF15 Trimer, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 10.2ng/ml determined by ELISA. The affinity constant of 3.06 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

N-His-Flag

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse DR3, His Tag immobilized on CM5 Chip can bind Human TNFSF15 (Trimer) , His Tag with an affinity constant of 3.06 nM as determined in SPR assay (Biacore T200).
Immobilized Human TNFSF15 Trimer, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 10.2ng/ml determined by ELISA.
The purity of Human TNFSF15 Trimer is greater than 95% as determined by SEC-HPLC.
Human TNFSF15 Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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