Human TNFSF15 Trimer Protein (FSF-HM415)

Express System: HEK293
Product Tag: N-His-Flag
Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Activity: Immobilized Human TNFSF15 Trimer, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA (QC Test). The affinity constant of 3.06 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Asp91-Leu251

Catalog: FSF-HM415

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Product Description	Recombinant Human TNFSF15 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus; It contains Asp91-Leu251.
Product Background	TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.
Product Category	Recombinant Protein

Target	TNFSF15 trimer
Synonyms	TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A
Accession	O95150-1
Species	Human
Amino Acid Range	Asp91-Leu251
Molecular Weight	The protein has a predicted MW of 58.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human TNFSF15 Trimer, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA (QC Test). The affinity constant of 3.06 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His-Flag
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Mouse DR3, His Tag immobilized on CM5 Chip can bind Human TNFSF15 (Trimer) , His Tag with an affinity constant of 3.06 nM as determined in SPR assay (Biacore T200).
	Immobilized Human TNFSF15 Trimer, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA (QC Test).
	The purity of Human TNFSF15 Trimer is greater than 95% as determined by SEC-HPLC.
	Human TNFSF15 Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.