Human TNFSF15 Trimer Protein (FSF-HM415)

  • Express System: HEK293
  • Product Tag: N-His-Flag
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human TNFSF15 Trimer, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA (QC Test). The affinity constant of 3.06 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Asp91-Leu251

Catalog: FSF-HM415

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Product Description Recombinant Human TNFSF15 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus; It contains Asp91-Leu251.

Product Background

TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.

Product Category

Recombinant Protein

Target

TNFSF15 trimer
Synonyms TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A
Accession

Species

Human

Amino Acid Range

Asp91-Leu251
Molecular Weight The protein has a predicted MW of 58.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human TNFSF15 Trimer, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA (QC Test). The affinity constant of 3.06 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

N-His-Flag

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1 EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse DR3, His Tag immobilized on CM5 Chip can bind Human TNFSF15 (Trimer) , His Tag with an affinity constant of 3.06 nM as determined in SPR assay (Biacore T200).
Immobilized Human TNFSF15 Trimer, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA (QC Test).
The purity of Human TNFSF15 Trimer is greater than 95% as determined by SEC-HPLC.
Human TNFSF15 Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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