Human Peptide Ready HLA-A*02:01&B2M Monomer-Protein (MHC-HM43R)
Human Peptide Ready HLA-A*02:01&B2M Monomer-Protein (MHC-HM43R)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Human Peptide Ready HLA-A*02:01&B2M Monomer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc tag with the EC50 of 2.5ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01) & Ile21-Met119(B2M)
Catalog #MHC-HM43R
Regular price $341.00
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Product Description

Recombinant Human Peptide Ready HLA-A*02:01&B2M Monomer-Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01) and Ile21-Met119(B2M).

Product Background

Peptide Ready HLA-A*02:01&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*02:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.

Product Category

Recombinant Protein / Antibody discovery / MHC

Protein

Peptide Ready HLA-A*02:01&B2M

Synonyms

MHC; HLA-A*02:01; Peptide Ready

MHC Form

Monomer

Allele

HLA-A*02:01

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Gly25-Thr305(HLA-A*02:01) & Ile21-Met119(B2M)

Molecular Weight

The protein has a predicted MW of 48.10 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human Peptide Ready HLA-A*02:01&B2M Monomer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc tag with the EC50 of 2.5ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22um filtered solution in PBS, 100mM L-arginine (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human Peptide Ready HLA-A*02:01&B2M Monomer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc tag with the EC50 of 2.5ng/ml determined by ELISA. Immobilized Human Peptide Ready HLA-A*02:01&B2M Monomer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc tag with the EC50 of 2.5ng/ml determined by ELISA.
Human Peptide Ready HLA-A*02:01&B2M Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Peptide Ready HLA-A*02:01&B2M Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Peptide Ready HLA-A*02:01&B2M Monomer is greater than 95% as determined by SEC-HPLC. The purity of Human Peptide Ready HLA-A*02:01&B2M Monomer is greater than 95% as determined by SEC-HPLC.

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