Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM205)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD3E&CD3D, hFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-CD3E&CD3D Ab.1, hFc Tag with the EC50 of 31.3ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D)
Catalog #CD3-HM205
Regular price $363.00
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Product Description

Recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from HEK293 with hFc at the C-Terminus of CD3E and CD3D.It contains Asp23-Asp126(CD3E) and Phe22-Ala105(CD3D).

Product Background

T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

CD3E&CD3D/CD3 epsilon&CD3 delta

Synonyms

CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D)

Molecular Weight

The protein has a predicted MW of 37.8 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation, the protein migrates to 48-58 kDa (CD3E) and 42-48 kDa (CD3D) based on Bis-Tris PAGE result.

Activity

Immobilized Human CD3E&CD3D, hFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-CD3E&CD3D Ab.1, hFc Tag with the EC50 of 31.3ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human CD3E&CD3D, hFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-CD3E&CD3D Ab.1, hFc Tag with the EC50 of 31.3ng/ml determined by ELISA. Immobilized Human CD3E&CD3D, hFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-CD3E&CD3D Ab.1, hFc Tag with the EC50 of 31.3ng/ml determined by ELISA.
Immobilized Human CD3E&CD3D, hFc Tag at 2ug/ml (100ul/Well). Dose response curve for Anti-CD3E&CD3D Ab.2, mFc Tag with the EC50 of 12.9ng/ml determined by ELISA (QC Test). Immobilized Human CD3E&CD3D, hFc Tag at 2ug/ml (100ul/Well). Dose response curve for Anti-CD3E&CD3D Ab.2, mFc Tag with the EC50 of 12.9ng/ml determined by ELISA (QC Test).
Immobilized Tebentafusp, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD3E&CD3D, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA. Immobilized Tebentafusp, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD3E&CD3D, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA.
Human CD3E&CD3D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD3E&CD3D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD3E&CD3D was greater than 95% as determined by SEC-HPLC. The purity of Human CD3E&CD3D was greater than 95% as determined by SEC-HPLC.

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