Name: Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM205)
Brand: KactusBio
SKU: CD3-HM205-100UG
Price: 363.0 USD
Availability: InStock

Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM205)

Immobilized Human CD3E&CD3D, hFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-CD3E&CD3D Ab.1, hFc Tag with the EC50 of 31.3ng/ml determined by ELISA.

Immobilized Human CD3E&CD3D, hFc Tag at 2ug/ml (100ul/Well). Dose response curve for Anti-CD3E&CD3D Ab.2, mFc Tag with the EC50 of 12.9ng/ml determined by ELISA (QC Test).

Immobilized Tebentafusp, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD3E&CD3D, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA.

Human CD3E&CD3D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human CD3E&CD3D was greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human CD3E&CD3D, hFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-CD3E&CD3D Ab.1, hFc Tag with the EC50 of 31.3ng/ml determined by ELISA.
Amino Acid Range: Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D)

Catalog #CD3-HM205

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM205) Summary

Product Description	Recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from HEK293 with hFc (IgG1) at the C-Terminus of CD3E and CD3D.It contains Asp23-Asp126(CD3E) and Phe22-Ala105(CD3D).
Product Background	T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM205) Specifications/Details

Protein	CD3E&CD3D/CD3 epsilon&CD3 delta
Synonyms	CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon
Accession	P07766(CD3E)&P04234(CD3D)
Species	Human
Biotinylated	no
Amino Acid Range	Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D)
Molecular Weight	The protein has a predicted MW of 37.8 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation, the protein migrates to 48-58 kDa (CD3E) and 42-48 kDa (CD3D) based on Bis-Tris PAGE result.
Activity	Immobilized Human CD3E&CD3D, hFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-CD3E&CD3D Ab.1, hFc Tag with the EC50 of 31.3ng/ml determined by ELISA.
Product Tag	C-hFc (IgG1)
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human CD3E&CD3D, hFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-CD3E&CD3D Ab.1, hFc Tag with the EC50 of 31.3ng/ml determined by ELISA.
	Immobilized Human CD3E&CD3D, hFc Tag at 2ug/ml (100ul/Well). Dose response curve for Anti-CD3E&CD3D Ab.2, mFc Tag with the EC50 of 12.9ng/ml determined by ELISA (QC Test).
	Immobilized Tebentafusp, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD3E&CD3D, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA.
	Human CD3E&CD3D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human CD3E&CD3D was greater than 90% as determined by SEC-HPLC.

Other Available Proteins:

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Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM205)

Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM205)

Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM205) Summary

Product Description

Product Background

Product Category

Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM205) Specifications/Details

Fluorophore

Shipping, Reconstitution, & Storage

Validation Images

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