Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM205)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD3E&CD3D, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3E&CD3D Ab.1, hFc Tag with the EC50 of 31.68ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D)

Catalog: CD3-HM205

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Product Description Recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from Expi293 with hFc at the C-terminal.It contains Asp23-Asp126(CD3E)&Phe22-Ala105(CD3D).
Product Category Recombinant Protein
Target CD3E&CD3D/CD3 epsilon&CD3 delta
Synonyms CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon
Gene/Protein ID P07766(CD3E)&P04234(CD3D)
Species Human
Exact Sequence Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D)
Molecular Weight The protein has a predicted MW of 37.8 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human CD3E&CD3D, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3E&CD3D Ab.1, hFc Tag with the EC50 of 31.68ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD3E&CD3D, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3E&CD3D Ab.1, hFc Tag with the EC50 of 31.68ng/ml determined by ELISA.
Immobilized Human CD3E&CD3D, hFc Tag at 0.1μg/ml (100ul/Well). Dose response curve for Anti-CD3E&CD3D Ab.2, mFc Tag with the EC50 of 27.0ng/ml determined by ELISA.
Immobilized Human CD3E&CD3D, hFc Tag at 1μg/ml (100μl/Well). Dose response curve for Anti-CD3E&CD3D Ab., mFc Tag (OKT3) with the EC50 of 28.8/25.5/27.8ng/ml determined by ELISA.
Immobilized Human CD3E&CD3D, hFc Tag at 1μg/ml (100μl/Well). Dose response curve for Anti-CD3E&CD3D Ab., mFc Tag (OKT3) with the EC50 of 23.8/22.6/23.5/22.8ng/ml determined by ELISA.
The purity of Human CD3E&CD3D was greater than 95% as determined by SEC-HPLC.
Human CD3E&CD3D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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