Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer-Protein (MHC-HM43RB)
Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer-Protein (MHC-HM43RB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01) & Ile21-Met119(B2M)
Catalog #MHC-HM43RB
Regular price $310.00
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Product Description
Recombinant Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01) and Ile21-Met119(B2M).
Product Background
HLA-A*02:01&B2M&Peptide ready Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*02:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
Product Category
Recombinant Protein
Target
Peptide Ready HLA-A*02:01&B2M Monomer
MHC Form
Monomer
Allele
HLA-A*02:01
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Gly25-Thr305(HLA-A*02:01) & Ile21-Met119(B2M)
Molecular Weight
The protein has a predicted MW of 48.10 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA.
Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer is greater than 90% as determined by SEC-HPLC.

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