Product Description |
Recombinant Biotinylated Human CDH6/Cadherin 6 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Thr19-Ala615. |
Product Background |
Cadherin 6 (CDH6) is an adhesion molecule localizing to the endometrial luminal epithelial cell surface in the mid-secretory/receptive phase and knockdown of CDH6 in the Ishikawa cells (receptive endometrial epithelial cell line) compromises cell integrity. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
CDH6/Cadherin 6 |
Synonyms |
Cadherin-6; Cadherin6; K-cadherin; CDH6; CAD6; KCAD; Kidney cadherin |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Thr19-Ala615 |
Molecular Weight |
The protein has a predicted MW of 69.29 kDa. Due to glycosylation, the protein migrates to 75-90 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human CDH6, His-Avi Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CDH6 Antibody, Rabbit IgG Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>90% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.； -80°C for 3 months after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
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Immobilized Biotinylated Human CDH6, His-Avi Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CDH6 Antibody, Rabbit IgG Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). |
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Biotinylated Human CDH6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Biotinylated Human CDH6 is greater than 90% as determined by SEC-HPLC. |
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