→ Choice of rapid procedure (2 hours) for fast turnaround or standard procedure (6 hours) for greater sensitivity
→ Specificity to AAV9 (no cross-reactivity with other AAV serotypes)
AAV9 Titration ELISA Kit
High specificity, sensitivity, and reproducibility
Empty AAV9 capsids lacking the transgene of interest caused unwanted immune response without providing the therapeutic benefit of the transgene. Alongside DNA and infectious titers, determination of the capsid titer is crucial for validating the efficacy and safety of your AAV gene therapy. Our titration kit detects full and empty AAV9 capsids.
KACTUS AAV9 ELISA kit has high specificity and sensitivity, with a wide linear range and strong reproducibility.
How it works
This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) to determine the quantity of AAV9 capsids in the test sample. The AAV monoclonal antibody is pre-coated on a 96-well reaction plate. AAV9 standard or sample is added to the pre-coated plate, which will specifically bind to the reaction plate. The biotinylated detection antibody is then added to bind the AAV9 capsids. Next, the HRP conjugate is added to form an antibody-antigen complex. HRP will then oxidize TMB substrate to produce a color change. The intensity of the color change is proportional to the AAV9 capsid titer in the sample. After reading the absorbance, the AAV9 capsid titer is calculated based on the absorbance values and the known AAV9 titers of the standard.
→ Sensitivity as low as 1.0E7 capsids/mL
→ Broad linear range: 1.0E7 - 6.E8 capsids/mL
→ Strong reproducibility
→ Low operation cost
Applications
→ Intact AAV wild type virions
→ AAV recombinant virions
→ Assembled AAV virions
→ Intact empty AAV capsids