Introduction
The streptavidin magnetic beads are superparamagnetic microspheres with uniform particle size and regular morphology. The beads are covalently coupled to streptavidin and blocked with BSA, which is ideal for capturing biotinylated proteins and other targets.
Applications
The streptavidin magnetic beads can be used to isolate biotinylated molecules, such as antigens and antibodies. They are suitable for immunocapture, phage display, biopanning, drug screening, and cell isolation.
For Research Use Only.
Product List and Specifications
| Specification | Streptavidin Magnetic Beads #MB-SA001 | Streptavidin Magnetic Beads for Cells #MB-SA002 | |
|---|---|---|---|
| Binding Capacity | Biotinylated IgG (μg/mg bead) | ≥15 | ≥15 |
| Biotinylated single-stranded oligonucleotide (24nt) (pmol/mg bead) | ≥350 | ≥450 | |
| Particle Size | 2μm | 300nm | |
| Concentration | 10 mg/ml | ||
| Surface | Hydrophilic | ||
| Sterility | No | Yes | |
| Suspension Medium | 1X PBS with 0.1% (w/v) BSA and 0.1% (v/v) ProClin 300 | ||
| Storage | 2°C to 8°C, avoid freezing or drying | ||
| Shelf Life | 2 years from date of manufacture | ||
| Browse Product | Order MB-SA001 | Order MB-SA002 | |
Product Features
High binding capacity provides rapid and efficient biomolecule capturing.
High-performance with non-aggregating, pre-blocked superparamagnetic microparticles
Fast and sensitive magnetic response
Adapts to different experimental conditions
Product Performance Data
Binding Capacity with Buffers
Figure 1. The binding capacity of streptavidin magnetic beads treated with different solutions.
Binding Capacity with Heat Treatment
Figure 2. The binding capacity of streptavidin magnetic beads after heat treatment.
Detection of Rabbit IgG
Figure 3. Streptavidin magnetic beads were used to detect biotinylated rabbit IgG. KACTUS beads enable highly sensitive detection of Biotinylated-IgG, with a limit of 6.25 ng/ml.
Detection of DNA Probe
Figure 4. Streptavidin magnetic beads were used to detect biotinylated DNA probe. KACTUS beads achieved highly sensitive detection of Biotinylated-DNA probes (LOD = 6.25 fmol/ml) with minimal nonspecific binding.
Frequently Asked Questions
Yes, we recommend MB-SA300C which is sterile.
The Streptavidin Magnetic Beads contain over 20 µg of streptavidin protein per mg of beads.
For MB-SA001, there are approximately 5 x 10^8 particles/ml of beads.
For MB-SA002, there are over 1 x 10^9 particles/ml of beads.
We do not recommend reusing the beads. The streptavidin-biotin bond is one of the strongest biological bonds known, and the conditions necessary to break this bond also destroy the streptavidin molecules.
For dissociating biotinylated DNA from Streptavidin magnetic beads, we recommend heating in 10 mM EDTA, 95% formamide, pH 8.2, for 5 min at 65 degrees C or for 2 min at 90 degrees C. Alternatively, the beads-DNA complex may be boiled for 5 min in 0.1% SDS.
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Waltham, MA 02451
United States
Streptavidin Magnetic Beads