Product Description |
Recombinant Biotinylated Human/Cynomolgus/Rhesus macaque ROR1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln30-Glu403. |
Product Background |
ROR1 (Receptor tyrosine kinase-like orphan receptor 1), also known as neurotrophic tyrosine kinase receptor-related 1 (NTRKR1),- is a member of- the ROR family within receptor tyrosine kinases (RTK) superfamily. Two ROR family members (ROR1 and ROR2) have been identified and are characterized by the intracellular tyrosine kinase domains, highly related to those of the Trk-family receptor tyrosine kinases, and by the extracellular Frizzled-like cysteine-rich domains and kringle domains, which are common to receptors of the Wnt family members. |
Product Category |
Recombinant Protein |
Target |
ROR1 |
Synonyms |
ROR1; NTRKR1; dJ537F10.1 |
Accession |
|
Species |
Human/Cynomolgus/Rhesus macaque |
Biotinylated |
yes |
Amino Acid Range |
Gln30-Glu403 |
Molecular Weight |
The protein has a predicted MW of 44.9 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human/Cynomolgus/Rhesus macaque ROR1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ROR1 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Biotinylated Human/Cynomolgus/Rhesus macaque ROR1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ROR1 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA. |
|
|
Biotinylated Human/Cynomolgus/Rhesus macaque ROR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Biotinylated Human/Cynomolgus/Rhesus macaque ROR1 is greater than 95% as determined by SEC-HPLC. |
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