Product Description |
Recombinant Human TPBG/5T4 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ser32-Ser355. |
Product Background |
The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs. |
Product Category |
Recombinant Protein |
Target |
TPBG/5T4 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser32-Ser355 |
Molecular Weight |
The protein has a predicted MW of 62.08 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human TPBG, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-TPBG Antibody, hFc Tag with the EC50 of 26.8ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human TPBG, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-TPBG Antibody, hFc Tag with the EC50 of 26.8ng/ml determined by ELISA. |
|
|
Human TPBG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human TPBG is greater than 95% as determined by SEC-HPLC. |
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