Name: Human TPBG/5T4 Protein (5T4-HM201)
Brand: KactusBio
SKU: 5T4-HM201-100UG
Price: 315.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human TPBG, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-TPBG Antibody, hFc Tag with the EC50 of 26.8ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Ser32-Ser355

Catalog #5T4-HM201

Size

100UG

500UG

Order now, ships by:

Product Description	Recombinant Human TPBG/5T4 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ser32-Ser355.
Product Background	The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs.
Product Category	Recombinant Protein / Antibody discovery

Protein	TPBG/5T4
Synonyms	M6P1; 5T4AG; WAIF1; TPBG; 5T4
Accession	Q13641
Species	Human
Biotinylated	no
Amino Acid Range	Ser32-Ser355
Molecular Weight	The protein has a predicted MW of 62.08 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human TPBG, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-TPBG Antibody, hFc Tag with the EC50 of 26.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human TPBG, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-TPBG Antibody, hFc Tag with the EC50 of 26.8ng/ml determined by ELISA.
	Human TPBG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human TPBG is greater than 95% as determined by SEC-HPLC.

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Product Description

Product Background