Human TPBG/5T4 Protein (5T4-HM401)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human TPBG, His Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 7.3 ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ser32-Ser355
Catalog #5T4-HM401
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Product Description
 Recombinant Human TPBG/5T4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus; It contains Ser32-Ser355.
Product Background
 The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs.
Product Category
 Recombinant Protein
Target
 TPBG/5T4
Synonyms
 M6P1; 5T4AG; WAIF1; TPBG; 5T4
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ser32-Ser355
Molecular Weight
 The protein has a predicted MW of 38.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human TPBG, His Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 7.3 ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human TPBG, His Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 7.3 ng/ml determined by ELISA.
Human TPBG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TPBG is greater than 95% as determined by SEC-HPLC.

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