Human TPBG/5T4 Protein (5T4-HM401)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human TPBG, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser32-Ser355

Catalog: 5T4-HM401

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Product Description Recombinant Human TPBG/5T4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser32-Ser355.

Product Background

The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs.

Product Category

Recombinant Protein

Target

TPBG/5T4
Synonyms M6P1; 5T4AG; WAIF1; TPBG; 5T4

Gene/Protein ID

Q13641-1

Species

Human

Exact Sequence

Ser32-Ser355
Molecular Weight The protein has a predicted MW of 38.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human TPBG, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human TPBG, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA.
The purity of Human TPBG is greater than 95% as determined by SEC-HPLC.
Human TPBG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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