Product Description |
Recombinant Human A2AR Protein-Nanodisc is expressed from HEK293 with His tag at the C-terminus.It contains Met1-Ser412. |
Product Background |
Adenosine is a neuromodulator in the adult central nervous system. Membrane-bound receptors for adenosine have been identified and cDNAs for A1, A2a, A2b, and A3 adenosine receptor subtypes have been cloned recently.Expression of A2a adenosine receptor mRNA in cranial ganglia, carotid body, and intermediate lobe of the pituitary gland similarly suggests novel sites of adenosine action during development and in the adult. |
Product Category |
Recombinant Protein |
Target |
A2AR |
Synonyms |
A2aR;Adenosine A2a R;ADORA 2;ADORA2;ADORA2A;RDC8 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met1-Ser412 |
Molecular Weight |
The protein has a predicted MW of 45.5 kDa. |
Activity |
Immobilized Human A2AR Nanodisc, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-A2AR Antibody, mFc Tag with the EC50 of 33.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.32 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS (pH 7.4). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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|
Human A2AR Nanodisc, His Tag captured on CM5 Chip via Anti-his antibody can bind Anti-A2AR Antibody, mFc Tag with an affinity constant of 0.32 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human A2AR Nanodisc, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-A2AR Antibody, mFc Tag with the EC50 of 33.1ng/ml determined by ELISA (QC Test). |
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Loaded Anti-A2AR Antibody on Protein A-Biosensor, can bind Human A2AR Nanodisc, His Tag with an affinity constant of 4.44 nM as determined in BLI assay (Gator). |
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