Human A2AR Nanodisc (A2R-HM1N1)

Express System: HEK293
Product Tag: C-His
Activity: Immobilized Human A2AR Nanodisc, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-A2AR Antibody, mFc Tag with the EC50 of 0.15ug/ml determined by ELISA. The affinity constant of 0.32 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Met1-Ser412

Catalog #A2R-HM1N1

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$1,210.00

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Human A2AR Nanodisc (A2R-HM1N1) Summary

Product Description	Recombinant Human A2AR Nanodisc is expressed from HEK293 with His tag at the C-terminus.It contains Met1-Ser412.
Product Background	Adenosine is a neuromodulator in the adult central nervous system. Membrane-bound receptors for adenosine have been identified and cDNAs for A1, A2a, A2b, and A3 adenosine receptor subtypes have been cloned recently.Expression of A2a adenosine receptor mRNA in cranial ganglia, carotid body, and intermediate lobe of the pituitary gland similarly suggests novel sites of adenosine action during development and in the adult.
Product Category	Recombinant Protein / Antibody discovery / Multiple transmembrane proteins / Nanodisc

Protein	A2AR
Synonyms	A2aR;Adenosine A2a R;ADORA 2;ADORA2;ADORA2A;RDC8
Accession	P29274
Species	Human
Biotinylated	no
Amino Acid Range	Met1-Ser412
Molecular Weight	The protein has a predicted MW of 45.5 kDa.
Activity	Immobilized Human A2AR Nanodisc, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-A2AR Antibody, mFc Tag with the EC50 of 0.15ug/ml determined by ELISA. The affinity constant of 0.32 nM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Stability And Storage	Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human A2AR Nanodisc, His Tag captured on CM5 Chip via Anti-his antibody can bind Anti-A2AR Antibody, mFc Tag with an affinity constant of 0.32 nM as determined in SPR assay (Biacore T200).
	Immobilized Human A2AR Nanodisc, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-A2AR Antibody, mFc Tag with the EC50 of 0.15ug/ml determined by ELISA.
	Loaded Anti-A2AR Antibody on Protein A-Biosensor, can bind Human A2AR Nanodisc, His Tag with an affinity constant of 4.44 nM as determined in BLI assay (Gator).

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