Human A2AR Protein-Nanodisc (A2R-HM1N1)

Express System: HEK293
Product Tag: C-His
Activity: Immobilized Human A2AR Nanodisc, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-A2AR Antibody, mFc Tag with the EC50 of 33.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.32 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Met1-Ser412

Catalog: A2R-HM1N1

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Product Description	Recombinant Human A2AR Protein-Nanodisc is expressed from HEK293 with His tag at the C-terminus.It contains Met1-Ser412.
Product Background	Adenosine is a neuromodulator in the adult central nervous system. Membrane-bound receptors for adenosine have been identified and cDNAs for A1, A2a, A2b, and A3 adenosine receptor subtypes have been cloned recently.Expression of A2a adenosine receptor mRNA in cranial ganglia, carotid body, and intermediate lobe of the pituitary gland similarly suggests novel sites of adenosine action during development and in the adult.
Product Category	Recombinant Protein

Target	A2AR
Gene/Protein ID	P29274
Species	Human
Exact Sequence	Met1-Ser412
Molecular Weight	The protein has a predicted MW of 45.5 kDa.
Activity	Immobilized Human A2AR Nanodisc, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-A2AR Antibody, mFc Tag with the EC50 of 33.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.32 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human A2AR Nanodisc, His Tag captured on CM5 Chip via Anti-his antibody can bind Anti-A2AR Antibody, mFc Tag with an affinity constant of 0.32 nM as determined in SPR assay (Biacore T200).
	Immobilized Human A2AR Nanodisc, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-A2AR Antibody, mFc Tag with the EC50 of 33.1ng/ml determined by ELISA (QC Test).
	Loaded Anti-A2AR Antibody on Protein A-Biosensor, can bind Human A2AR Nanodisc, His Tag with an affinity constant of 4.44 nM as determined in BLI assay (Gator).