AAV5 Titration ELISA Kit (AV5-MM00B)
SKU: AV5-MM00B-96T
AAV5 Titration ELISA Kit (AV5-MM00B)
SKU: AV5-MM00B-96T
AAV5 Titration ELISA Kit (AV5-MM00B) Summary
Product Description |
Detection Principle: This kit uses sandwich ELISA to determine the titer of AAV5 capsids in the test sample. The capture AAV5 monoclonal antibody is pre-coated and treated on a 96-well reaction plate. AAV5 standard or sample is added to the pre-coated plate and specifically binds to the capture antibody. The biotinylated detection AAV5 antibody is then added to the plate to bind the immune complex. Next, the streptavidin HRP conjugate is added to react with the biotin molecules. The addition of TMB results in color changes and the amplitude of the color change is proportional to the amount of AAV5 capsids that specifically bind to the plate. The reaction is stopped with the addition of stop solution and the absorbance is measured at 450nm. The sample AAV5 capsid titer is calculated from the AAV5 standards titration curve. |
AAV5 Titration ELISA Kit (AV5-MM00B) Specifications/Details
Applications |
Intact AAV5 Wild Type Virions AAV5 Recombinant Virions Assembled AAV5 Virions Intact Empty AAV5 Capsids |
Quality Standards |
Detection Range: 3.13E+07 capsids/mL - 2E+09 capsids/mL Sensitivity: 1.5E+07 capsids/mL |
Shipping, Reconstitution, & Storage
Form |
Multi |
Stability And Storage |
Store kit components at 2-8°C. The kit is valid for 12 months from the production date. |
Validation Images
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Figure 1. Example standard curve. |
Other Available Proteins:
Finding available proteins...
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