Product Description |
Recombinant Biotinylated Cynomolgus Fc gamma RIII/CD16 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly17-Gln208. |
Product Background |
Immunoglobulin G (IgG) Fc receptors play a critical role in linking IgG antibody-mediated immune responses with cellular effector functions. A high resolution map of the binding site on human IgG1 for human Fc gamma RI, Fc gamma RIIA, Fc gamma RIIB, Fc gamma RIIIA, and FcRn receptors has been determined.A common set of IgG1 residues is involved in binding to all Fc gamma R; Fc gamma RII and Fc gamma RIII also utilize residues outside this common set. |
Product Category |
Recombinant Protein |
Target |
Fc gamma RIII/CD16 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
yes |
Amino Acid Range |
Gly17-Gln208 |
Molecular Weight |
The protein has a predicted MW of 24.86 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.15 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Biotinylated Cynomolgus Fc gamma RIII, His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab with an affinity constant of 0.15 uM as determined in SPR assay (Biacore T200) (QC Test). |
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Biotinylated Cynomolgus Fc gamma RIII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Cynomolgus Fc gamma RIII is greater than 95% as determined by SEC-HPLC. |
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