Biotinylated Human APOE3/Apolipoprotein E Protein (Primary Amine Labeling) (APO-HM101B)
Catalog: APO-HM101B
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| Product Description | Recombinant Biotinylated Human APOE3/Apolipoprotein E Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the N-Terminus.It contains Lys19-His317. |
| Product Category | Recombinant Protein |
| Target | APOE3/Apolipoprotein E |
| Synonyms | Apolipoprotein E; Apo-E; APOE; apolipo E |
| Gene/Protein ID | P02649-1 |
| Species | Human |
| Exact Sequence | Lys19-His317 |
| Molecular Weight | The protein has a predicted MW of 35.3 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result. |
| Activity | The affinity constant of 1.41 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | N-His |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human TREM2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human APOE3, His Tag with an affinity constant of 1.41 uM as determined in SPR assay (Biacore T200). |
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Biotinylated Human APOE3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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