Biotinylated Human APOE3/Apolipoprotein E Protein (Primary Amine Labeling) (APO-HM101B)

  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: The affinity constant of 1.41 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Lys19-His317

Catalog: APO-HM101B

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Product Description Recombinant Biotinylated Human APOE3/Apolipoprotein E Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the N-Terminus.It contains Lys19-His317.

Product Background

 Apolipoprotein E (apoE) is a lipid carrier in both the peripheral and the central nervous systems. Lipid-loaded apoE lipoprotein particles bind to several cell surface receptors to support membrane homeostasis and injury repair in the brain. Considering prevalence and relative risk magnitude, the ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer's disease (AD).

Product Category

 Recombinant Protein

Target

 APOE3/Apolipoprotein E
Synonyms Apolipoprotein E; Apo-E; APOE; apolipo E

Gene/Protein ID

 P02649-1

Species

 Human

Exact Sequence

 Lys19-His317
Molecular Weight The protein has a predicted MW of 35.3 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.

Activity

 The affinity constant of 1.41 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

 N-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human TREM2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human APOE3, His Tag with an affinity constant of 1.41 uM as determined in SPR assay (Biacore T200).
Biotinylated Human APOE3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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