Name: Biotinylated Human APOE3/Apolipoprotein E Protein (Primary Amine Labeling) (APO-HM101B)
Brand: KactusBio
SKU: APO-HM101B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human APOE3/Apolipoprotein E Protein (Primary Amine Labeling) (APO-HM101B)

Human TREM2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human APOE3, His Tag with an affinity constant of 1.41 uM as determined in SPR assay (Biacore T200).

Biotinylated Human APOE3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: The affinity constant of 1.41 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Lys19-His317

Catalog #APO-HM101B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human APOE3/Apolipoprotein E Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the N-Terminus.It contains Lys19-His317.
Product Background	Apolipoprotein E (apoE) is a lipid carrier in both the peripheral and the central nervous systems. Lipid-loaded apoE lipoprotein particles bind to several cell surface receptors to support membrane homeostasis and injury repair in the brain. Considering prevalence and relative risk magnitude, the ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer's disease (AD).
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	APOE3/Apolipoprotein E
Synonyms	Apolipoprotein E; Apo-E; APOE; apolipo E; APOE3
Accession	P02649-1
Species	Human
Biotinylated	yes
Amino Acid Range	Lys19-His317
Molecular Weight	The protein has a predicted MW of 35.3 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 1.41 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human TREM2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human APOE3, His Tag with an affinity constant of 1.41 uM as determined in SPR assay (Biacore T200).
	Biotinylated Human APOE3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.