Biotinylated Human APOE3/Apolipoprotein E Protein (Primary Amine Labeling) (APO-HM101B)

  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: The affinity constant of 1.41 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Lys19-His317

Catalog: APO-HM101B

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Product Description Recombinant Biotinylated Human APOE3/Apolipoprotein E Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the N-Terminus.It contains Lys19-His317.
Product Category Recombinant Protein
Target APOE3/Apolipoprotein E
Synonyms Apolipoprotein E; Apo-E; APOE; apolipo E
Gene/Protein ID P02649-1
Species Human
Exact Sequence Lys19-His317
Molecular Weight The protein has a predicted MW of 35.3 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 1.41 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag N-His
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human TREM2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human APOE3, His Tag with an affinity constant of 1.41 uM as determined in SPR assay (Biacore T200).
Biotinylated Human APOE3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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