Biotinylated Human CD3E/CD3 epsilon Protein (Primary Amine Labeling) (CDE-HM101B)
Biotinylated Human CD3E/CD3 epsilon Protein (Primary Amine Labeling) (CDE-HM101B)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Anti-CD3 Antibody, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD3E, His Tag with the EC50 of 1.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Asp23-Asp126
Catalog #CDE-HM101B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human CD3E/CD3 epsilon Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Asp23-Asp126.
Product Background
CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.
Product Category
Recombinant Protein
Target
CD3E/CD3 epsilon
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Asp23-Asp126
Molecular Weight
The protein has a predicted MW of 12.6 kDa. Due to glycosylation, the protein migrates to 14-18 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Anti-CD3 Antibody, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD3E, His Tag with the EC50 of 1.0ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-CD3 Antibody, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD3E, His Tag with the EC50 of 1.0ng/ml determined by ELISA.
Biotinylated Human CD3E on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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