Biotinylated Human CD40 Ligand/TNFSF5 Trimer Protein (Primary Amine Labeling) (CDL-HM140B)
Catalog: CDL-HM140B
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| Product Description | Recombinant Biotinylated Human CD40 Ligand/TNFSF5 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Met113-Leu261. |
Product Background |
CD40 ligand or CD40L, also called CD154,- is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells. |
Product Category |
Recombinant Protein |
Target |
CD40 Ligand/TNFSF5 Trimer |
| Synonyms | CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand |
Gene/Protein ID |
P29965 |
Species |
Human |
Exact Sequence |
Met113-Leu261 |
| Molecular Weight | The protein has a predicted MW of 52.4 kDa, Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Biotinylated Human CD40 Ligand Trimer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD40, hFc Tag with the EC50 of 0.61ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His-Flag |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Biotinylated Human CD40 Ligand Trimer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD40, hFc Tag with the EC50 of 0.61ug/ml determined by ELISA. |
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The purity of Biotinylated Human CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human CD40 Ligand Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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