Biotinylated Human CD40 Ligand/TNFSF5 Trimer Protein (Primary Amine Labeling) (CDL-HM140B)

  • Express System: Expi293
  • Product Tag: N-His-Flag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human CD40 Ligand Trimer, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human CD40, hFc Tag with the EC50 of 0.61μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Met113-Leu261

Catalog: CDL-HM140B

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Product Description Recombinant Biotinylated Human CD40 Ligand/TNFSF5 Trimer Protein (Primary Amine Labeling) is expressed from Expi293 with His tag and Flag tag at the N-terminal.It contains Met113-Leu261.
Product Category Recombinant Protein
Target CD40 Ligand/TNFSF5 Trimer
Synonyms CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand
Gene/Protein ID P29965
Species Human
Exact Sequence Met113-Leu261
Molecular Weight The protein has a predicted MW of 52.4 kDa, Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Human CD40 Ligand Trimer, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human CD40, hFc Tag with the EC50 of 0.61μg/ml determined by ELISA. See testing image for detail.
Product Tag N-His-Flag
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Liquid
Shipping Shipped with blue ice.
Formulation Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
Immobilized Biotinylated Human CD40 Ligand Trimer, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human CD40, hFc Tag with the EC50 of 0.61μg/ml determined by ELISA.
The purity of Biotinylated Human CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC.
Biotinylated Human CD40 Ligand Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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