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Biotinylated Human CXCR5 Nanodisc (CXC-HM1RNB) Summary
Product Description |
Recombinant Biotinylated Human CXCR5 Nanodisc is expressed from HEK293 with His tag at the C-terminus.It contains Met1-Phe372. |
Product Background |
CXCR5 is a serpentine receptor implicated in cell migration in lymphocytes and differentiation in leukocytes. It causes MAPK pathway activation and has known membrane partners for signaling. CXCR5 is also expressed in HL-60 cells, a human acute myeloid leukemia line, following treatment with all-trans retinoic acid, which induces differentiation toward a neutrophil-like state. CXCR5 is necessary for this process; differentiation was crippled in CXCR5 knockout cells and enhanced in cells ectopically expressing it. |
Product Category |
Recombinant Protein / Antibody discovery / Multiple transmembrane proteins / Nanodisc |
Biotinylated Human CXCR5 Nanodisc (CXC-HM1RNB) Specifications/Details
Protein |
CXCR5 |
Synonyms |
CXC-R5; CXCR-5; MDR-15; MDR15; CD185; BLR1 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Met1-Phe372 |
Molecular Weight |
The protein has a predicted MW of 54.9 kDa. |
Activity |
Immobilized Biotinylated Human CXCR5 Nanodisc, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CXCR5 Antibody, hFc Tag with the EC50 of 13.7ng/ml determined by ELISA. The affinity constant of 15.36 pM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4). |
Stability And Storage |
Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Biotinylated Human CXCR5 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-CXCR5 Antibody, hFc Tag with an affinity constant of 15.36 pM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Biotinylated Human CXCR5 Nanodisc, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CXCR5 Antibody, hFc Tag with the EC50 of 13.7ng/ml determined by ELISA. |
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