• Express System: HEK293
  • Product Tag: C-His
  • Activity: Immobilized Biotinylated Human CXCR5 Nanodisc, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CXCR5 Antibody, hFc Tag with the EC50 of 13.7ng/ml determined by ELISA. The affinity constant of 15.36 pM as determined in SPR assay (Biacore T200).
  • Negative Control: Biotinylated Nanodisc Control (CON-HM1NB)
  • Amino Acid Range: Met1-Phe372
Catalog #CXC-HM1RNB
Price
Regular price $2,16590
Regular price Sale price $2,16590
/
Size
Avantor VWR Fisher Scientific

Order by 4pm ET for delivery on:

Biotinylated Human CXCR5 Nanodisc (CXC-HM1RNB) Summary

Product Description

 Recombinant Biotinylated Human CXCR5 Nanodisc is expressed from HEK293 with His tag at the C-terminus.It contains Met1-Phe372.

Product Background

 CXCR5 is a serpentine receptor implicated in cell migration in lymphocytes and differentiation in leukocytes. It causes MAPK pathway activation and has known membrane partners for signaling. CXCR5 is also expressed in HL-60 cells, a human acute myeloid leukemia line, following treatment with all-trans retinoic acid, which induces differentiation toward a neutrophil-like state. CXCR5 is necessary for this process; differentiation was crippled in CXCR5 knockout cells and enhanced in cells ectopically expressing it.

Biotinylated Human CXCR5 Nanodisc (CXC-HM1RNB) Specifications/Details

Protein
 CXCR5
Synonyms
 CXC-R5; CXCR-5; MDR-15; MDR15; CD185; BLR1;CXCR5
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Met1-Phe372
Molecular Weight
 The protein has a predicted MW of 54.9 kDa.
Activity
 Immobilized Biotinylated Human CXCR5 Nanodisc, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CXCR5 Antibody, hFc Tag with the EC50 of 13.7ng/ml determined by ELISA. The affinity constant of 15.36 pM as determined in SPR assay (Biacore T200).
Product Tag
 C-His
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Stability And Storage
 Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Biotinylated Human CXCR5 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-CXCR5 Antibody, hFc Tag with an affinity constant of 15.36 pM as determined in SPR assay (Biacore T200). Biotinylated Human CXCR5 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-CXCR5 Antibody, hFc Tag with an affinity constant of 15.36 pM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human CXCR5 Nanodisc, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CXCR5 Antibody, hFc Tag with the EC50 of 13.7ng/ml determined by ELISA. Immobilized Biotinylated Human CXCR5 Nanodisc, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CXCR5 Antibody, hFc Tag with the EC50 of 13.7ng/ml determined by ELISA.
Other Available Proteins:
Finding available proteins...