Name: Biotinylated Human CXCR5 Nanodisc (CXC-HM1RNB)
Brand: KactusBio
SKU: CXC-HM1RNB-100UG
Price: 2165.9 USD
Availability: InStock

Biotinylated Human CXCR5 Nanodisc (CXC-HM1RNB)

Biotinylated Human CXCR5 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-CXCR5 Antibody, hFc Tag with an affinity constant of 15.36 pM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Human CXCR5 Nanodisc, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CXCR5 Antibody, hFc Tag with the EC50 of 13.7ng/ml determined by ELISA.

Express System: HEK293
Product Tag: C-His
Activity: Immobilized Biotinylated Human CXCR5 Nanodisc, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CXCR5 Antibody, hFc Tag with the EC50 of 13.7ng/ml determined by ELISA. The affinity constant of 15.36 pM as determined in SPR assay (Biacore T200).
Amino Acid Range: Met1-Phe372

Catalog #CXC-HM1RNB

Size

100UG

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Biotinylated Human CXCR5 Nanodisc (CXC-HM1RNB) Summary

Product Description	Recombinant Biotinylated Human CXCR5 Nanodisc is expressed from HEK293 with His tag at the C-terminus.It contains Met1-Phe372.
Product Background	CXCR5 is a serpentine receptor implicated in cell migration in lymphocytes and differentiation in leukocytes. It causes MAPK pathway activation and has known membrane partners for signaling. CXCR5 is also expressed in HL-60 cells, a human acute myeloid leukemia line, following treatment with all-trans retinoic acid, which induces differentiation toward a neutrophil-like state. CXCR5 is necessary for this process; differentiation was crippled in CXCR5 knockout cells and enhanced in cells ectopically expressing it.
Product Category	Recombinant Protein / Antibody discovery / Multiple transmembrane proteins / Nanodisc

Biotinylated Human CXCR5 Nanodisc (CXC-HM1RNB) Specifications/Details

Protein	CXCR5
Synonyms	CXC-R5; CXCR-5; MDR-15; MDR15; CD185; BLR1
Accession	P32302-1
Species	Human
Biotinylated	yes
Amino Acid Range	Met1-Phe372
Molecular Weight	The protein has a predicted MW of 54.9 kDa.
Activity	Immobilized Biotinylated Human CXCR5 Nanodisc, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CXCR5 Antibody, hFc Tag with the EC50 of 13.7ng/ml determined by ELISA. The affinity constant of 15.36 pM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Expression System	HEK293
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Stability And Storage	Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Biotinylated Human CXCR5 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-CXCR5 Antibody, hFc Tag with an affinity constant of 15.36 pM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human CXCR5 Nanodisc, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CXCR5 Antibody, hFc Tag with the EC50 of 13.7ng/ml determined by ELISA.