Biotinylated Human FGFR1 alpha (IIIc) Protein (FGF-HM4RAB)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-FGFR1 Anitibody, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human FGFR1 alpha (IIIc) , His Tag with the EC50 of 25.9ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Arg22-Glu374

Catalog: FGF-HM4RAB

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Product Description Recombinant Biotinylated Human FGFR1 alpha (IIIc) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Arg22-Glu374.
Product Category Recombinant Protein
Target FGFR1 alpha (IIIc)
Synonyms FGF R1a; FGFR1 alpha
Gene/Protein ID P11362-7
Species Human
Exact Sequence Arg22-Glu374
Molecular Weight The protein has a predicted MW of 42 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-BisPAGE result.
Activity Immobilized Anti-FGFR1 Anitibody, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human FGFR1 alpha (IIIc) , His Tag with the EC50 of 25.9ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-FGFR1 Anitibody, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human FGFR1 alpha (IIIc) , His Tag with the EC50 of 25.9ng/ml determined by ELISA.
The purity of Biotinylated Human FGFR1 alpha (IIIc) is greater than 95% as determined by SEC-HPLC.
Biotinylated Human FGFR1 alpha (IIIc)  on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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