Biotinylated Human FGFR1 alpha (IIIc) Protein (FGF-HM4RAB)
Biotinylated Human FGFR1 alpha (IIIc) Protein (FGF-HM4RAB)
Biotinylated Human FGFR1 alpha (IIIc) Protein (FGF-HM4RAB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human FGFR1 alpha (IIIc), His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FGFR1 Antibody, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Arg22-Glu374
Catalog #FGF-HM4RAB
Regular price $577.50
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Biotinylated Human FGFR1 alpha (IIIc) Protein (FGF-HM4RAB) Summary

Product Description

Recombinant Biotinylated Human FGFR1 alpha (IIIc) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg22-Glu374.

Product Background

Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding.FGFR1 is tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Biotinylated Human FGFR1 alpha (IIIc) Protein (FGF-HM4RAB) Specifications/Details

Protein
FGFR1 alpha (IIIc)
Synonyms
FGF R1a; FGFR1 alpha
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Arg22-Glu374
Molecular Weight
The protein has a predicted MW of 42 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-BisPAGE result.
Activity
Immobilized Biotinylated Human FGFR1 alpha (IIIc), His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FGFR1 Antibody, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human FGFR1 alpha (IIIc), His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FGFR1 Antibody, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA. Immobilized Biotinylated Human FGFR1 alpha (IIIc), His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FGFR1 Antibody, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA.
Biotinylated Human FGFR1 alpha (IIIc) -on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human FGFR1 alpha (IIIc) -on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated-Human FGFR1 alpha (IIIc) is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated-Human FGFR1 alpha (IIIc) is greater than 95% as determined by SEC-HPLC.
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