Biotinylated Human HLA-G Complex Tetramer Protein (HLG-HM41CTB)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: The affinity constant of 4.38 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gly25-Thr305(HLA-G),Ile21-Met119(B2M)and RIIPRHLQL peptide

Catalog: HLG-HM41CTB

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Product Description Recombinant Biotinylated Human HLA-G Complex Tetramer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-G),Ile21-Met119(B2M) and RIIPRHLQL peptide.
Product Category Recombinant Protein
Target HLA-G Complex Tetramer
Synonyms HLA G antigen; sHLA-G; b2 microglobulin; HLA G; HLAG; HLA-G; MHC Class I Antigen G; MHC class Ib antigen; MHC-G; sHLA-G
Gene/Protein ID P17693-1(HLA-G)&P61769(B2M)&RIIPRHLQL
Species Human
Exact Sequence Gly25-Thr305(HLA-G),Ile21-Met119(B2M)and RIIPRHLQL peptide
Molecular Weight The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
Activity The affinity constant of 4.38 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human HLA-G Complex Tetramer, His-Avi Tag with an affinity constant of 4.38 nM as determined in SPR assay (Biacore T200).
Serial dilutions of Anti-LILRB2 Antibody were added into Human LILRB2, His Tag : Biotinylated HLA-G Complex Tetramer, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 75.3ng/ml.
The purity of Biotinylated Human HLA-G Complex Tetramer is greater than 95% as determined by SEC-HPLC.
Biotinylated Human HLA-G Complex Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.

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