Name: Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein (HLG-HM41CTB)
Brand: KactusBio
SKU: HLG-HM41CTB-100UG
Price: 1856.8 USD
Availability: InStock

Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein (HLG-HM41CTB)

Human LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His-Avi Tag with an affinity constant of 4.38 nM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-G Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA (QC Test).

Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%.

Serial dilutions of Anti-LILRB2 Antibody were added into Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag: Human LILRB2, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.29ug/ml.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-G Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA (QC Test). The affinity constant of 4.38 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gly25-Thr305(HLA-G), Ile21-Met119(B2M) and RIIPRHLQL peptide

Catalog #HLG-HM41CTB

Size

100UG

500UG

Order now, ships by:

[{"id":42618362134750,"title":"100UG","option1":"100UG","option2":null,"option3":null,"sku":"HLG-HM41CTB-100UG","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Biotinylated Human HLA-G\u0026B2M\u0026Peptide (RIIPRHLQL) Tetramer Protein (HLG-HM41CTB) - 100UG","public_title":"100UG","options":["100UG"],"price":185680,"weight":5,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}},{"id":43584255852766,"title":"500UG","option1":"500UG","option2":null,"option3":null,"sku":"HLG-HM41CTB-500UG","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Biotinylated Human HLA-G\u0026B2M\u0026Peptide (RIIPRHLQL) Tetramer Protein (HLG-HM41CTB) - 500UG","public_title":"500UG","options":["500UG"],"price":618750,"weight":5,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}}]

Product Description	Recombinant Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-G), Ile21-Met119(B2M) and RIIPRHLQL peptide.
Product Background	HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition.
Product Category	Recombinant Protein / Antibody discovery / MHC

Protein	HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer
Synonyms	HLA G antigen; sHLA-G; b2 microglobulin; HLA G; HLAG; HLA-G; MHC Class I Antigen G; MHC class Ib antigen; MHC-G; sHLA-G
MHC Form	Tetramer
Allele	HLA-G
Accession	P17693-1(HLA-G)&P61769(B2M)&RIIPRHLQL
Species	Human
Biotinylated	yes
Amino Acid Range	Gly25-Thr305(HLA-G), Ile21-Met119(B2M) and RIIPRHLQL peptide
Molecular Weight	The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-G Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA (QC Test). The affinity constant of 4.38 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His-Avi Tag with an affinity constant of 4.38 nM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-G Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA (QC Test).
	Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%.
	Serial dilutions of Anti-LILRB2 Antibody were added into Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag: Human LILRB2, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.29ug/ml.