Biotinylated Human IL-17A&F Protein (ILF-HM418B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human IL-17R alpha, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human IL-17A&F, His Tag with the EC50 of 0.22ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gly24-Ala155(IL-17A) & Arg31-Gln163(IL-17F)

Catalog: ILF-HM418B

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Product Description Recombinant Biotinylated Human IL-17A&F Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly24-Ala155(IL-17A)&Arg31-Gln163(IL-17F).
Product Category Recombinant Protein
Target IL-17A&F
Synonyms IL-17A;Interleukin-17A;CTLA-8;IL-17;IL-17F;IL24;ML-1;Interleukin-17F
Gene/Protein ID Q16552-1(IL-17A)&Q96PD4(IL-17F)
Species Human
Exact Sequence Gly24-Ala155(IL-17A) & Arg31-Gln163(IL-17F)
Molecular Weight The protein has a predicted MW of 15.1 kDa (IL-17A)&17.5 kDa (IL-17F). Due to glycosylation, the protein migrates to 16 kDa&27 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human IL-17R alpha, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human IL-17A&F, His Tag with the EC50 of 0.22ug/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-17R alpha, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human IL-17A&F, His Tag with the EC50 of 0.22ug/ml determined by ELISA.
Biotinylated Human IL-17A&F on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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