Biotinylated Human IL-17A&F Protein (ILF-HM418B)
Catalog: ILF-HM418B
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| Product Description | Recombinant Biotinylated Human IL-17A&F Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gly24-Ala155(IL-17A)&Arg31-Gln163(IL-17F). |
| Product Category | Recombinant Protein |
| Target | IL-17A&F |
| Synonyms | IL-17A；Interleukin-17A；CTLA-8；IL-17；IL-17F；IL24；ML-1；Interleukin-17F |
| Gene/Protein ID | Q16552-1(IL-17A)&Q96PD4(IL-17F) |
| Species | Human |
| Exact Sequence | Gly24-Ala155(IL-17A) & Arg31-Gln163(IL-17F) |
| Molecular Weight | The protein has a predicted MW of 15.1 kDa (IL-17A)&17.5 kDa (IL-17F). Due to glycosylation, the protein migrates to 16 kDa&27 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human IL-17R alpha, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human IL-17A&F, His Tag with the EC50 of 0.22μg/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human IL-17R alpha, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human IL-17A&F, His Tag with the EC50 of 0.22μg/ml determined by ELISA. |
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Biotinylated Human IL-17A&F on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. |
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