Biotinylated Human IL-17A&F Protein (ILF-HM418B)
Catalog: ILF-HM418B
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Product Description | Recombinant Biotinylated Human IL-17A&F Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly24-Ala155(IL-17A)&Arg31-Gln163(IL-17F). |
Product Category | Recombinant Protein |
Target | IL-17A&F |
Synonyms | IL-17A;Interleukin-17A;CTLA-8;IL-17;IL-17F;IL24;ML-1;Interleukin-17F |
Gene/Protein ID | Q16552-1(IL-17A)&Q96PD4(IL-17F) |
Species | Human |
Exact Sequence | Gly24-Ala155(IL-17A) & Arg31-Gln163(IL-17F) |
Molecular Weight | The protein has a predicted MW of 15.1 kDa (IL-17A)&17.5 kDa (IL-17F). Due to glycosylation, the protein migrates to 16 kDa&27 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human IL-17R alpha, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human IL-17A&F, His Tag with the EC50 of 0.22ug/ml determined by ELISA. See testing image for detail. |
Product Tag | C-His-Avi |
Express System | HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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