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Biotinylated Human LAIR2/CD306 Protein (LAI-HM4R2B) Summary
Product Description |
Recombinant Biotinylated Human LAIR2/CD306 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln22-Pro152. |
Product Background |
The leukocyte-associated immunoglobulin-like receptor 2 (LAIR2) shows increased expression during pregnancy and its reduced expression is associated with pregnancy complications. Although LAIR2 was expressed in the human placenta villus and decidua in the first trimester of pregnancy, it was not expressed in mouse tissues. Knockdown of LAIR2 markedly improved cell viability and inhibited the invasive ability of HTR8/SVneo cells. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Biotinylated Human LAIR2/CD306 Protein (LAI-HM4R2B) Specifications/Details
Protein |
LAIR2/CD306 |
Synonyms |
LAIR-2; CD306; LAIR2; MGC71634; XXbac-BCX535A19.6 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gln22-Pro152 |
Molecular Weight |
The protein has a predicted MW of 17 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 1.80 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Biotinylated Human LAIR2, His-Avi Tag immobilized on CM5 Chip can bind Native Human Collagen I, No Tag with an affinity constant of 1.80 nM as determined in SPR assay (Biacore T200). |
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Biotinylated Huma LAIR2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Huma LAIR2 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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