Name: Biotinylated Human Peptide Ready HLA-B*07:02&B2M Monomer Protein (MHC-HM47RB)
Brand: KactusBio
SKU: MHC-HM47RB-100UG
Price: 341.0 USD
Availability: InStock

Biotinylated Human Peptide Ready HLA-B*07:02&B2M Monomer Protein (MHC-HM47RB)

Immobilized Biotinylated Human Peptide Ready HLA-B*07:02&B2M Monomer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA.

Biotinylated Human Peptide Ready HLA-B*07:02&B2M Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human Peptide Ready HLA-B*07:02&B2M Monomer is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Biotinylated Human Peptide Ready HLA-B*07:02&B2M Monomer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA.
Amino Acid Range: Gly25-Val309(HLA-B*07:02) & Ile21-Met119(B2M)

Catalog #MHC-HM47RB

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human Peptide Ready HLA-B*07:02&B2M Monomer Protein (MHC-HM47RB) Summary

Product Description	Recombinant Biotinylated Human Peptide Ready HLA-B07:02&B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Val309 (HLA-B07:02) and Ile21-Met119 (B2M).
Product Background	Peptide Ready HLA-B07:02&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-B07:02. Peptide ready MHC molecules comprising human HLA-B*07:02 alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
Product Category	Recombinant Protein / Antibody discovery / MHC / Peptide-ready MHCs

Peptide Ready HLA-B*07:02&B2M Specifications

Protein	Peptide Ready HLA-B*07:02&B2M
Synonyms	MHC; HLA-B; Peptide Ready
MHC Form	Monomer
Allele	HLA-B*07:02
Accession	P01889(HLA-B*07:02)&P61769(B2M)
Species	Human
Biotinylated	yes
Amino Acid Range	Gly25-Val309(HLA-B*07:02) & Ile21-Met119(B2M)
Molecular Weight	The protein has a predicted MW of 48.50 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human Peptide Ready HLA-B*07:02&B2M Monomer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS, 100mM L-arginine (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human Peptide Ready HLA-B*07:02&B2M Monomer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA.
	Biotinylated Human Peptide Ready HLA-B*07:02&B2M Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human Peptide Ready HLA-B*07:02&B2M Monomer is greater than 90% as determined by SEC-HPLC.