Biotinylated Human PSMA/FOLH1 Protein (PSM-HM410B)
  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Biotinylated Human PSMA, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-PSMA Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Lys44-Ala750
Catalog #PSM-HM410B
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Product Description
 Recombinant Biotinylated Human PSMA/FOLH1 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Lys44-Ala750.
Product Background
 Prostate-specific membrane antigen (PSMA) is an enzyme that in humans is encoded by the FOLH1 (folate hydrolase 1) gene, also known as Glutamate carboxypeptidase II (GCPII). Human PSMA is highly expressed in the prostate, roughly a hundred times greater than in most other tissues. In some prostate cancers, PSMA is the second-most upregulated gene product, with an 8- to 12-fold increase over levels in noncancerous prostate cells.
Product Category
 Recombinant Protein
Target
 PSMA/FOLH1
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Lys44-Ala750
Molecular Weight
 The protein has a predicted MW of 82.43 kDa. Due to glycosylation, the protein migrates to 95-115 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human PSMA, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-PSMA Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. See testing image for detail.
Product Tag
 N-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 25 mM MES, 500 mM NaCl (pH 6.5). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human PSMA, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-PSMA Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA.
Biotinylated Human PSMA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human PSMA is greater than 90% as determined by SEC-HPLC.

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