Product Description |
Recombinant Biotinylated Human PSMA/FOLH1 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Lys44-Ala750. |
Product Background |
Prostate-specific membrane antigen (PSMA) is an enzyme that in humans is encoded by the FOLH1 (folate hydrolase 1) gene, also known as Glutamate carboxypeptidase II (GCPII). Human PSMA is highly expressed in the prostate, roughly a hundred times greater than in most other tissues. In some prostate cancers, PSMA is the second-most upregulated gene product, with an 8- to 12-fold increase over levels in noncancerous prostate cells. |
Product Category |
Recombinant Protein |
Protein |
PSMA/FOLH1 |
Synonyms |
GCP2; PSMA; PSMAFGCP; FGCP; Folate hydrolase 1; FOLHNAALADase I; GCPII; GCPIImGCP; NAALAD1; NAALADase I; NAALAdase; FOLH1; GIG27; PSM; FOLH; mGCP |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Lys44-Ala750 |
Molecular Weight |
The protein has a predicted MW of 82.43 kDa. Due to glycosylation, the protein migrates to 95-115 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human PSMA, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-PSMA Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
N-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 25 mM MES, 500 mM NaCl (pH 6.5). Normally 8% mannitol is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Biotinylated Human PSMA, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-PSMA Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test). |
|
|
Biotinylated Human PSMA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Biotinylated Human PSMA is greater than 90% as determined by SEC-HPLC. |
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