Biotinylated Human PSMA/FOLH1 Protein (PSM-HM410B)

  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human PSMA, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-PSMA Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Lys44-Ala750

Catalog: PSM-HM410B

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Product Description Recombinant Biotinylated Human PSMA/FOLH1 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Lys44-Ala750.

Product Background

 Prostate-specific membrane antigen (PSMA) is an enzyme that in humans is encoded by the FOLH1 (folate hydrolase 1) gene, also known as Glutamate carboxypeptidase II (GCPII). Human PSMA is highly expressed in the prostate, roughly a hundred times greater than in most other tissues. In some prostate cancers, PSMA is the second-most upregulated gene product, with an 8- to 12-fold increase over levels in noncancerous prostate cells.

Product Category

 Recombinant Protein

Target

 PSMA/FOLH1
Accession

Species

 Human

Amino Acid Range

 Lys44-Ala750
Molecular Weight The protein has a predicted MW of 82.43 kDa. Due to glycosylation, the protein migrates to 95-115 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Biotinylated Human PSMA, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-PSMA Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

 N-His-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in 25 mM MES, 500 mM NaCl (pH 6.5). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human PSMA, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-PSMA Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA.
The purity of Biotinylated Human PSMA is greater than 90% as determined by SEC-HPLC.
Biotinylated Human PSMA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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