Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-CM101)
Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-CM101)
  • Express System: HEK293
  • Product Tag: C-His-Flag
  • Purity: >95% as determined by Bis-Tris PAGE; >95% as determined by HPLC
  • Activity: Immobilized Cynomolgus CD3E&CD3D, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 5.6 ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gln22-Asp117 (CD3E) & Phe22-Ala105 (CD3D)
Catalog #CD3-CM101
Regular price $300.00
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Product Description
Recombinant Cynomolgus CD3E&CD3D or CD3 epsilon&CD3 delta protein is expressed from HEK293 with His tag and Flag tag at the C-Terminus.It contains Gln22-Asp117 (CD3E)&Phe22-Ala105 (CD3D).
Product Background
T-cell surface glycoprotein CD3 epsilon&CD3 delta chain, also known as CD3E&CD3D, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Product Category
Recombinant Protein
Target
CD3E&CD3D/CD3 epsilon&CD3 delta
Synonyms
CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Gln22-Asp117 (CD3E) & Phe22-Ala105 (CD3D)
Molecular Weight
The protein has a predicted MW of 11.93 kDa (CD3E) and 10.55 kDa (CD3D). Due to glycosylation, the protein migrates to 13-20 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus CD3E&CD3D, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 5.6 ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
C-His-Flag
Express System
HEK293
Purity
>95% as determined by Bis-Tris PAGE; >95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus CD3E&CD3D, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 5.6 ng/ml determined by ELISA (QC Test).
Cynomolgus CD3E&CD3D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus CD3E&CD3D is greater than 95% as determined by SEC-HPLC.

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