Cynomolgus DNAM-1/CD226 Protein (DAM-CM101)
Cynomolgus DNAM-1/CD226 Protein (DAM-CM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus DNAM-1, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 1.63ug/ml determined by ELISA (QC Test). The affinity constant of 0.82 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Glu19-Phe252
Catalog #DAM-CM101
Regular price $315.00
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Product Description
Recombinant Cynomolgus DNAM-1/CD226 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu19-Phe252.
Product Background
DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.
Product Category
Recombinant Protein
Target
DNAM-1/CD226
Synonyms
CD226 molecule; CD226; DNAM1; PTA1; TLiSA1
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Glu19-Phe252
Molecular Weight
The protein has a predicted MW of 27.7 kDa. Due to glycosylation, the protein migrates to 48-68 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus DNAM-1, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 1.63ug/ml determined by ELISA (QC Test). The affinity constant of 0.82 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human CD155, hFc Tag- captured on CM5 Chip via Protein A can bind Cynomolgus DNAM-1, His Tag with an affinity constant of 0.82 uM as determined in SPR assay (Biacore T200).
Immobilized Cynomolgus DNAM-1, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 1.63ug/ml determined by ELISA (QC Test).
Cynomolgus DNAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus DNAM-1 is greater than 95% as determined by SEC-HPLC.

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