Cynomolgus DNAM-1/CD226 Protein (DAM-CM101)
Catalog: DAM-CM101
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| Product Description | Recombinant Cynomolgus DNAM-1/CD226 Protein is expressed from Expi293 with His tag at the C-terminal.; It contains Glu19-Phe252. |
| Product Category | Recombinant Protein |
| Target | DNAM-1/CD226 |
| Synonyms | CD226 molecule; CD226; DNAM1; PTA1; TLiSA1 |
| Gene/Protein ID | A0A2K5W1Z6-1 |
| Species | Cynomolgus |
| Exact Sequence | Glu19-Phe252 |
| Molecular Weight | The protein has a predicted MW of 27.7 kDa. Due to glycosylation, the protein migrates to 48-68 kDa based on Tris-Bis PAGE result. |
| Activity | The affinity constant of 0.881 μM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus DNAM-1, His Tag with an affinity constant of 0.881 μM as determined in SPR assay (Biacore T200). |
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The purity of Cynomolgus DNAM-1 is greater than 95% as determined by SEC-HPLC. |
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Cynomolgus DNAM-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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