Cynomolgus DNAM-1/CD226 Protein (DAM-CM101)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: The affinity constant of 0.881 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Glu19-Phe252

Catalog: DAM-CM101

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Product Description Recombinant Cynomolgus DNAM-1/CD226 Protein is expressed from Expi293 with His tag at the C-terminal.; It contains Glu19-Phe252.
Product Category Recombinant Protein
Target DNAM-1/CD226
Synonyms CD226 molecule; CD226; DNAM1; PTA1; TLiSA1
Gene/Protein ID A0A2K5W1Z6-1
Species Cynomolgus
Exact Sequence Glu19-Phe252
Molecular Weight The protein has a predicted MW of 27.7 kDa. Due to glycosylation, the protein migrates to 48-68 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 0.881 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus DNAM-1, His Tag with an affinity constant of 0.881 μM as determined in SPR assay (Biacore T200).
The purity of Cynomolgus DNAM-1 is greater than 95% as determined by SEC-HPLC.
Cynomolgus DNAM-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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