Cynomolgus M-CSF/CSF-1 Protein (CSF-CM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Cynomolgus M-CSF, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.889 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Glu33-Leu255
Catalog #CSF-CM101
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Product Description
 Recombinant Cynomolgus M-CSF/CSF-1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu33-Leu255.
Product Background
 CSF-1, also known as M-CSF , is a four alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. CSF-1 is also essential for the survival and proliferation of osteoclast progenitors.CSF-1 is cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.
Product Category
 Recombinant Protein
Target
 M-CSF/CSF-1
Synonyms
 CSF1; CSF-1; MCSF; M-CSF; MGC31930; Lanimostim
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Glu33-Leu255
Molecular Weight
 The protein has a predicted MW of 26.26 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus M-CSF, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.889 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Cynomolgus M-CSF R, His Tag immobilized on CM5 Chip can bind Cynomolgus M-CSF, His Tag with an affinity constant of 0.889 uM as determined in SPR assay (Biacore T200).
Immobilized Cynomolgus M-CSF, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA (QC Test).
Cynomolgus M-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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