Cynomolgus PD-1/PDCD1 Protein (PD1-CM201)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus PD-1, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PD1 Antibody, hFc Tag with the EC50 of 29.5ng/ml determined by ELISA (QC Test). The affinity constant of 1.28 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Pro21-Gln167

Catalog: PD1-CM201

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Product Description	Recombinant Cynomolgus PD-1/PDCD1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Pro21-Gln167.
Product Background	Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity.
Product Category	Recombinant Protein

Target	PD-1/PDCD1
Synonyms	PDCD1; PD1; CD279; SLEB2; PD-1
Gene/Protein ID	B0LAJ3
Species	Cynomolgus
Exact Sequence	Pro21-Gln167
Molecular Weight	The protein has a predicted MW of 42.4 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus PD-1, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PD1 Antibody, hFc Tag with the EC50 of 29.5ng/ml determined by ELISA (QC Test). The affinity constant of 1.28 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Cynomolgus PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus PD-L1, His Tag with an affinity constant of 1.28 uM as determined in SPR assay (Biacore T200).
	Immobilized Cynomolgus PD-1, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PD1 Antibody, hFc Tag with the EC50 of 29.5ng/ml determined by ELISA (QC Test).
	The purity of Cynomolgus PD-1 is greater than 95% as determined by SEC-HPLC.
	Cynomolgus PD-1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.