Cynomolgus PD-1/PDCD1 Protein (PD1-CM201)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus PD-1, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PD1 Antibody, hFc Tag with the EC50 of 29.5ng/ml determined by ELISA (QC Test). The affinity constant of 1.28 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Pro21-Gln167

Catalog: PD1-CM201

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Product Description Recombinant Cynomolgus PD-1/PDCD1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Pro21-Gln167.

Product Background

Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity.

Product Category

Recombinant Protein

Target

PD-1/PDCD1
Synonyms PDCD1; PD1; CD279; SLEB2; PD-1
Accession

Species

Cynomolgus

Amino Acid Range

Pro21-Gln167
Molecular Weight The protein has a predicted MW of 42.4 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Cynomolgus PD-1, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PD1 Antibody, hFc Tag with the EC50 of 29.5ng/ml determined by ELISA (QC Test). The affinity constant of 1.28 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Cynomolgus PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus PD-L1, His Tag with an affinity constant of 1.28 uM as determined in SPR assay (Biacore T200).
Immobilized Cynomolgus PD-1, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PD1 Antibody, hFc Tag with the EC50 of 29.5ng/ml determined by ELISA (QC Test).
The purity of Cynomolgus PD-1 is greater than 95% as determined by SEC-HPLC.
Cynomolgus PD-1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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