DNase I, RNase-free, GMP grade (GMP-DNI-EE001)
Catalog: GMP-DNI-EE001
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Product Description | DNase I is an endonuclease that digests single- and double-stranded DNA to oligonucleotide containing a 5' phosphate and 3'-hydroxylated ends. It is an essential enzyme for the efficient digestion of DNA during RNA purification and is suitable for molecular diagnostics and in vitro mRNA synthesis applications. |
Application | In Vitro Transcription (IVT) Removal of DNA from RNA samples DNase I footprinting |
Concentration | 4U/µL |
Unit Definition | One unit corresponds to the amount of enzyme required to produce a change in absorbance at 260 nm of 1.0 in 10 minutes. |
Source | Expressed in an E.coli strain with DNase I gene isolated from bovine pancreas. |
Molecular Weight | 39kD |
Quality Standards | Activity (Degradation plasmid method): ≥ 4 kU/mL
Purity (SEC-HPLC): ≥ 95% Residual RNase: Negative Residual Protease: Negative Endotoxin: ≤ 10 EU/mL Residual Host Cell DNA: ≤ 100 pg/mg Residual Host protein: ≤ 20 ng/mg Residual Heavy Metal: ≤ 10 ppm Bioburden: ≤ 1 CFU/10 mL |
Form | Liquid |
Stability And Storage | -20C±5°C for 12 months. |
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