DNaseI (RNase-free), GMP Grade (GMP-DNI-EE001)
Catalog: GMP-DNI-EE001
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| Product Description | DNase I is an endonuclease that digests single- and double-stranded DNA to oligonucleotide containing a 5' phosphate and 3'-hydroxylated ends. Both single- and double-stranded DNA are degraded by DNaseI. It is an essential enzyme for the efficient digestion of DNA during RNA purification, suitable for all molecular diagnostics and in vitro mRNA synthesis applications. Expressed in an E.coli strain with DNaseI gene isolated from bovine pancreas. |
| Product Category | GMP Enzyme |
| Application | In Vitro Synthesis(IVT) Removal of DNA from RNA samples DNase I footprinting |
| Concentration | 4 U/UL |
| Unit Definition | One unit is defined as the amount of enzyme which will completely degrade 1μg of pBR322 DNA in 10 minutes at 37°C in DNase I Reaction Buffer. |
| Molecular Weight | 39 kD |
| Express System | E.coli |
| Quality Statement | Manufactured to the GMP guidelines High purity and tested for contaminating RNase and protease activities Suitable for the isolation of DNA-free RNA in diagnostic and mRNA therapeutics applications |
| QC Standard | Activity: >=4 kU/ml
Purity: >=95% Residual RNase: Negative Residual Protease: Negative Endotoxin: <=10 EU/ml Residual Host Cell DNA: <=100 pg/MG Residual Host protein: <=50 ng/MG Bioburden: <=1 CFU/10 ml |
| Form | Liquid |
| Shipping | Shipped with blue ice |
| Stability And Storage | -20°C for 12 months. Avoid repeated freeze-thaw cycles. |
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Fig.1 Add 1μg DNA and different of DNaseI in a total reaction volume of 10μl. Compared with competitor 1 and 2, DNase I of kactus has a stronger digestive effect. |
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