DNase I, RNase-free, GMP grade (GMP-DNI-EE001)
Catalog: GMP-DNI-EE001
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| Product Description | DNase I is an endonuclease that digests single- and double-stranded DNA to oligonucleotide containing a 5' phosphate and 3'-hydroxylated ends. It is an essential enzyme for the efficient digestion of DNA during RNA purification and is suitable for molecular diagnostics and in vitro mRNA synthesis applications. |
| Application | In Vitro Transcription (IVT) Removal of DNA from RNA samples DNase I footprinting |
| Concentration | 4U/µL |
| Unit Definition | One unit corresponds to the amount of enzyme required to produce a change in absorbance at 260 nm of 1.0 in 10 minutes. |
| Source | Expressed in an E.coli strain with DNase I gene isolated from bovine pancreas. |
| Molecular Weight | 39kD |
| Quality Standards | Activity (Degradation plasmid method): ≥ 4 kU/mL
Purity (SEC-HPLC): ≥ 95% Residual RNase: Negative Residual Protease: Negative Endotoxin: ≤ 10 EU/mL Residual Host Cell DNA: ≤ 100 pg/mg Residual Host protein: ≤ 20 ng/mg Residual Heavy Metal: ≤ 10 ppm Bioburden: ≤ 1 CFU/10 mL |
| Form | Liquid |
| Stability And Storage | -20C±5°C for 12 months. |
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Figure 1. 1µg DNA was added to various activity units of DNase I in a total reaction volume of 10µL. Compared with competitors 1 and 2, DNase I of KACTUS has a stronger digestive effect. |
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Figure 2. Different concentrations of ions were added to the transcriptional reaction. Results show DNase I is inhibited by Na+ and K+. |
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