• Description: Digests single- and double-stranded DNA to oligonucleotides containing a 5' phosphate and 3'-hydroxylated ends
  • Applications: In Vitro Transcription (IVT)
    Removal of DNA from RNA samples
    DNase I footprinting
  • Quality: FDA Drug Master Files #038032
    Manufactured according to GMP guidelines
    High purity and tested for contaminating RNase and protease activities
    Suitable for the isolation of DNA-free RNA in diagnostic and mRNA therapeutics applications
Catalog #GMP-DNI-EE001
Price
Regular price $481.80
Regular price Sale price $481.80
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DNase I, RNase-free, GMP-Grade (GMP-DNI-EE001), DMF #038032 Summary

Product Description

 DNase I is an endonuclease that digests single- and double-stranded DNA to oligonucleotide containing a 5' phosphate and 3'-hydroxylated ends. It is an essential enzyme for the efficient digestion of DNA during RNA purification and is suitable for molecular diagnostics and in vitro mRNA synthesis applications.

This product has been submitted to the FDA Drug Master Files and is assigned DMF #038032.

DNase I, RNase-free, GMP-Grade (GMP-DNI-EE001), DMF #038032 Specifications/Details

Synonyms
 DNase1; DNase 1; DNaseI; Deoxyribonuclease I; DeoxyribonucleaseI; Deoxyribonuclease 1; Deoxyribonuclease1;

Applications

 In Vitro Transcription (IVT)
Removal of DNA from RNA samples
DNase I footprinting

Concentration

 4U/µL

Unit Definition

 One unit is the amount of enzyme required to completely degrade 1 µg of pBR322 DNA in 10 minutes at 37 °C.

Source

 Expressed in an E.coli strain with DNase I gene isolated from bovine pancreas.
Biotinylated
 no

Quality Standards

 Activity (Degradation plasmid method): 4.0~8.0 kU/mL
Purity (SEC-HPLC): ≥ 95%
Residual RNase: Negative
Residual Protease: Negative
Endotoxin: ≤ 1.2 EU/mL
Residual Heavy Metal: ≤ 10 ppm
Residual Nickel Salt: ≤ 10 ppm
Bioburden: ≤ 1 CFU/10 mL
Concentration: 0.190 mg/ml±20%

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipping with dry ice
Stability And Storage
 -20C±5°C for 36 months.

Validation Images

Figure 1. 1µg DNA was added to various activity units of DNase I in a total reaction volume of 10µL. Compared with competitors 1 and 2, DNase I of KACTUS has a stronger digestive effect. Figure 1. 1µg DNA was added to various activity units of DNase I in a total reaction volume of 10µL. Compared with competitors 1 and 2, DNase I of KACTUS has a stronger digestive effect.
Figure 2. Different concentrations of ions were added to the transcriptional reaction. Results show DNase I is inhibited by Na+ and K+. Figure 2. Different concentrations of ions were added to the transcriptional reaction. Results show DNase I is inhibited by Na+ and K+.
Other Available Proteins:
Finding available proteins...
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support@kactusbio.us

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