DNase I, RNase-free, GMP grade (GMP-DNI-EE001)

Catalog: GMP-DNI-EE001

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Product Description DNase I is an endonuclease that digests single- and double-stranded DNA to oligonucleotide containing a 5' phosphate and 3'-hydroxylated ends. It is an essential enzyme for the efficient digestion of DNA during RNA purification and is suitable for molecular diagnostics and in vitro mRNA synthesis applications.
Application In Vitro Transcription (IVT)
Removal of DNA from RNA samples
DNase I footprinting
Concentration 4U/µL
Unit Definition One unit corresponds to the amount of enzyme required to produce a change in absorbance at 260 nm of 1.0 in 10 minutes.
Source Expressed in an E.coli strain with DNase I gene isolated from bovine pancreas.
Molecular Weight 39kD
Quality Standards Activity (Degradation plasmid method): ≥ 4 kU/mL
Purity (SEC-HPLC): ≥ 95%
Residual RNase: Negative
Residual Protease: Negative
Endotoxin: ≤ 10 EU/mL
Residual Host Cell DNA: ≤ 100 pg/mg
Residual Host protein: ≤ 20 ng/mg
Residual Heavy Metal: ≤ 10 ppm
Bioburden: ≤ 1 CFU/10 mL
Form Liquid
Stability And Storage -20C±5°C for 12 months.
Figure 1. 1µg DNA was added to various activity units of DNase I in a total reaction volume of 10µL. Compared with competitors 1 and 2, DNase I of KACTUS has a stronger digestive effect.
Figure 2. Different concentrations of ions were added to the transcriptional reaction. Results show DNase I is inhibited by Na+ and K+.

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