Mouse Her2/ErbB2 Protein (HER-MM102)

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse Her2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HER2 Antibody, Rabbit IgG Tag with the EC50 of 33.9ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Thr23-Thr653

Catalog: HER-MM102

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Product Description	Recombinant Mouse Her2/ErbB2 Protein is expressed from HEK293 with His tag at the N-terminus.It contains Thr23-Thr653.
Product Background	ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors.Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane.
Product Category	Recombinant Protein

Target	Her2/ErbB2
Gene/Protein ID	P70424
Species	Mouse
Exact Sequence	Thr23-Thr653
Molecular Weight	The protein has a predicted MW of 70.74 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Mouse Her2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HER2 Antibody, Rabbit IgG Tag with the EC50 of 33.9ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Mouse Her2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HER2 Antibody, Rabbit IgG Tag with the EC50 of 33.9ng/ml determined by ELISA.
	The purity of Mouse Her2 is greater than 95% as determined by SEC-HPLC.
	Mouse Her2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.