- Express System: HEK293
- Product Tag: N-His
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Mouse Her2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HER2 Antibody, Rabbit Fc Tag with the EC50 of 33.3ng/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Thr23-Thr653
Product Description |
Recombinant Mouse Her2/ErbB2 Protein is expressed from HEK293 with His tag at the N-terminus.It contains Thr23-Thr653. |
Product Background |
ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors.Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. |
Product Category |
Recombinant Protein |
Target |
Her2/ErbB2 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Thr23-Thr653 |
Molecular Weight |
The protein has a predicted MW of 70.74 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse Her2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HER2 Antibody, Rabbit Fc Tag with the EC50 of 33.3ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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