Mouse Her2/ErbB2 Protein (HER-MM102)
Mouse Her2/ErbB2 Protein (HER-MM102)
Mouse Her2/ErbB2 Protein (HER-MM102)
Mouse Her2/ErbB2 Protein (HER-MM102)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse Her2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HER2 Antibody, Rabbit Fc Tag with the EC50 of 33.3ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Thr23-Thr653
Catalog #HER-MM102
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Product Description
 Recombinant Mouse Her2/ErbB2 Protein is expressed from HEK293 with His tag at the N-terminus.It contains Thr23-Thr653.
Product Background
 ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors.Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane.
Product Category
 Recombinant Protein
Target
 Her2/ErbB2
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Thr23-Thr653
Molecular Weight
 The protein has a predicted MW of 70.74 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Mouse Her2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HER2 Antibody, Rabbit Fc Tag with the EC50 of 33.3ng/ml determined by ELISA. See testing image for detail.
Product Tag
 N-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse Her2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HER2 Antibody, Rabbit Fc Tag with the EC50 of 33.3ng/ml determined by ELISA.
Mouse Her2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse Her2 is greater than 95% as determined by SEC-HPLC.
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