Human B7-H2/ICOSLG Protein (BH7-HM272)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human ICOS, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 25.3ng/ml determined by ELISA (QC Test). The affinity constant of 40.2nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Asp19-Ser258

Catalog: BH7-HM272

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Product Description Recombinant Human B7-H2/ICOSLG Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Asp19-Ser258.

Product Background

B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells.

Product Category

Recombinant Protein

Target

B7-H2/ICOSLG
Synonyms CD275; B7H2; B7-H2; B7RP-1; GL50; ICOSL; ICOS-L; ICOSLG; B7RP1; LICOS; ICOS ligand; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50
Accession

Species

Human

Amino Acid Range

Asp19-Ser258
Molecular Weight The protein has a predicted MW of 53.4 kDa. Due to glycosylation, the protein migrates to 75-100 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human ICOS, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 25.3ng/ml determined by ELISA (QC Test). The affinity constant of 40.2nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human B7-H2 captured on Protein A chip, can bind Human ICOS (C136S,C137S) , His Tag with an affinity constant of 40.2nM as determined in a SPR assay (Biacore T200).
Immobilized Human ICOS, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 25.3ng/ml determined by ELISA (QC Test).
The purity of Human B7-H2 is greater than 95% as determined by SEC-HPLC.
Human B7-H2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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