Name: Human B7-H2/ICOSLG Protein (BH7-HM272)
Brand: KactusBio
SKU: BH7-HM272-100UG
Price: 270.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human ICOS, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 25.3ng/ml determined by ELISA (QC Test). The affinity constant of 40.2nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Asp19-Ser258

Catalog #BH7-HM272

Size

100UG

500UG

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Product Description	Recombinant Human B7-H2/ICOSLG Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Asp19-Ser258.
Product Background	B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	B7-H2/ICOSLG
Synonyms	CD275; B7H2; B7-H2; B7RP-1; GL50; ICOSL; ICOS-L; ICOSLG; B7RP1; LICOS; ICOS ligand; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50
Accession	O75144-1
Species	Human
Biotinylated	no
Amino Acid Range	Asp19-Ser258
Molecular Weight	The protein has a predicted MW of 53.4 kDa. Due to glycosylation, the protein migrates to 75-100 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human ICOS, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 25.3ng/ml determined by ELISA (QC Test). The affinity constant of 40.2nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human B7-H2 captured on Protein A chip, can bind Human ICOS (C136S,C137S) , His Tag with an affinity constant of 40.2nM as determined in a SPR assay (Biacore T200).
	Immobilized Human ICOS, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 25.3ng/ml determined by ELISA (QC Test).
	Human B7-H2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human B7-H2 is greater than 95% as determined by SEC-HPLC.