Human CDCP1 ATF (30-368) Protein (CDC-HM11D)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CDCP1 ATF (30-368), His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Phe30-Arg368
Catalog #CDC-HM11D
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Product Description
 Recombinant Human CDCP1 ATF (30-368) Protein is expressed from HEK293 with His tag at the N-terminus.It contains Phe30-Arg368.
Product Background
 Tumor metastasis depends on the dynamic regulation of cell adhesion through β1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression.
Product Category
 Recombinant Protein
Target
 CDCP1 ATF (30-368)
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Phe30-Arg368
Molecular Weight
 The protein has a predicted MW of 39.03 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CDCP1 ATF (30-368), His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA. See testing image for detail.
Product Tag
 N-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CDCP1 ATF (30-368), His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.
Human CDCP1 ATF (30-368) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CDCP1 ATF (30-368) is greater than 95% as determined by SEC-HPLC.

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