Human CDCP1 ATF (30-368) Protein (CDC-HM11D)

  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CDCP1 ATF (30-368), His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Phe30-Arg368

Catalog: CDC-HM11D

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Product Description Recombinant Human CDCP1 ATF (30-368) Protein is expressed from HEK293 with His tag at the N-terminus.It contains Phe30-Arg368.

Product Background

Tumor metastasis depends on the dynamic regulation of cell adhesion through β1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression.

Product Category

Recombinant Protein

Target

CDCP1 ATF (30-368)
Accession

Species

Human

Amino Acid Range

Phe30-Arg368
Molecular Weight The protein has a predicted MW of 39.03 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human CDCP1 ATF (30-368), His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CDCP1 ATF (30-368), His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.
The purity of Human CDCP1 ATF (30-368) is greater than 95% as determined by SEC-HPLC.
Human CDCP1 ATF (30-368) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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