Human Fc gamma RIIIA/CD16a (V176) Protein (FCR-HM43A)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Fc gamma RIIIA (V176), His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA (QC test). The affinity constant of 67.3nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly17-Gln208(V176)
Catalog #FCR-HM43A
Regular price $240.00
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Product Description
Recombinant Human Fc gamma RIIIA/CD16a (V176) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly17-Gln208(V176).
Product Background
Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
Product Category
Recombinant Protein
Target
Fc gamma RIIIA/CD16a (V176)
Synonyms
CD16FCRIIIA; Fc fragment of IgG; Fc gamma RIIIA; Fc-gamma RIII-alpha; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gly17-Gln208(V176)
Molecular Weight
The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human Fc gamma RIIIA (V176), His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA (QC test). The affinity constant of 67.3nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Fc gamma RIIIA (V176) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Herceptin, hFc Tag with an affinity constant of 67.3nM as determined in SPR assay (Biacore T200).
Immobilized Human Fc gamma RIIIA (V176), His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA (QC test).
Human-Fc gamma RIIIA (V176) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Fc gamma RIIIA (V176) is greater than 95% as determined by SEC-HPLC.

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