Human FGFR2 beta (IIIb) Domain Protein (FGF-HM2BD)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human FGFR2 beta (IIIb) Domain, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 14.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Pro253-Glu378

Catalog: FGF-HM2BD

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Product Description Recombinant Human FGFR2 beta (IIIb) Domain Protein is expressed from Expi293 with hFc tag at the C-terminal.; It contains Pro253-Glu378.
Product Category Recombinant Protein
Target FGFR2 beta (IIIb) Domain
Synonyms Fibroblast growth factor receptor 2; FGFR-2; KGFR; K-sam; Keratinocyte growth factor receptor; CD332; BEK; KSAM
Gene/Protein ID P21802-3
Species Human
Exact Sequence Pro253-Glu378
Molecular Weight The protein has a predicted MW of 40.5 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human FGFR2 beta (IIIb) Domain, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 14.3ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human FGFR2 beta (IIIb) Domain, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 14.3ng/ml determined by ELISA.
The purity of Human FGFR2 beta (IIIb) Domain is greater than 95% as determined by SEC-HPLC.
Human FGFR2 beta (IIIb) Domain on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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