• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human FGFR2 beta (IIIb) Domain, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 30.7ng/ml determined by ELISA.
  • Amino Acid Range: Pro253-Glu378
Catalog #FGF-HM2BD
Price
Regular price $379.50
Regular price Sale price $379.50
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Size
Avantor VWR Fisher Scientific

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Human FGFR2 beta (IIIb) Domain Protein (FGF-HM2BD) Summary

Product Description

 Recombinant Human FGFR2 beta (IIIb) Domain Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Pro253-Glu378.

Product Background

 Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the beta isoform.

Human FGFR2 beta (IIIb) Domain Protein (FGF-HM2BD) Specifications/Details

Protein
 FGFR2 beta (IIIb)
Synonyms
 Fibroblast growth factor receptor 2;FGFR-2;KGFR;K-sam;Keratinocyte growth factor receptor;CD332;BEK;KSAM;FGFR2
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Pro253-Glu378
Molecular Weight
 The protein has a predicted MW of 40.5 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human FGFR2 beta (IIIb) Domain, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 30.7ng/ml determined by ELISA.
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human FGFR2 beta (IIIb) Domain, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 30.7ng/ml determined by ELISA. Immobilized Human FGFR2 beta (IIIb) Domain, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 30.7ng/ml determined by ELISA.
Human FGFR2 beta (IIIb) Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human FGFR2 beta (IIIb) Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human FGFR2 beta (IIIb) Domain is greater than 95% as determined by SEC-HPLC. The purity of Human FGFR2 beta (IIIb) Domain is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...