• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human FGFR2 beta (IIIb), His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FGFR2 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test). The affinity constant of 1.98nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Arg152-Glu378
Catalog #FGR-HM1BB
Price
Regular price $379.50
Regular price Sale price $379.50
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Size
Avantor VWR Fisher Scientific

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Human FGFR2 beta (IIIb) Protein (FGR-HM1BB) Summary

Product Description

 Recombinant Human FGFR2 beta (IIIb) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg152-Glu378.

Product Background

 Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha- isoform, or only IgII and IgIII, referred to as the beta- isoform.

Human FGFR2 beta (IIIb) Protein (FGR-HM1BB) Specifications/Details

Protein
 FGFR2 beta (IIIb)
Synonyms
 Fibroblast growth factor receptor 2;FGFR-2;KGFR;K-sam;Keratinocyte growth factor receptor;CD332;BEK;KSAM;FGFR2
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Arg152-Glu378
Molecular Weight
 The protein has a predicted MW of 26.5 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human FGFR2 beta (IIIb), His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FGFR2 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test). The affinity constant of 1.98nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Anti-FGFR2 Antibody, hFc Tag can bind Human FGFR2 beta (IIIb) , His Tag with an affinity constant of 1.98nM as determined in a SPR assay (Biacore T200). Anti-FGFR2 Antibody, hFc Tag can bind Human FGFR2 beta (IIIb) , His Tag with an affinity constant of 1.98nM as determined in a SPR assay (Biacore T200).
Immobilized Human FGFR2 beta (IIIb), His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FGFR2 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test). Immobilized Human FGFR2 beta (IIIb), His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FGFR2 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test).
Human FGFR2 beta (IIIb) on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Human FGFR2 beta (IIIb) on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
Serial dilutions of Anti-FGFR2 Antibody were added into Human FGFR2 beta (lllb) , His Tag : Biotinylated Human FGF10, No Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 1.74ng/ml. Serial dilutions of Anti-FGFR2 Antibody were added into Human FGFR2 beta (lllb) , His Tag : Biotinylated Human FGF10, No Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 1.74ng/ml.
The purity of Human FGFR2 beta (IIIb) is greater than 95% as determined by SEC-HPLC. The purity of Human FGFR2 beta (IIIb) is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...